Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24394 | 5' | -54 | NC_005264.1 | + | 126101 | 0.67 | 0.959184 |
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Target: 5'- cCGGcGCCGUCGcgcUGCGGGGa----- -3' miRNA: 3'- aGCCuCGGCAGCu--ACGCCUCauaugc -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 136649 | 0.66 | 0.962698 |
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Target: 5'- gCGGAGCC-UCGAcgcgcGUGGAGUAa--- -3' miRNA: 3'- aGCCUCGGcAGCUa----CGCCUCAUaugc -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 59807 | 0.66 | 0.962698 |
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Target: 5'- gCGGAGCCGUacguaGAcGUGaGGGUcgcGUACGc -3' miRNA: 3'- aGCCUCGGCAg----CUaCGC-CUCA---UAUGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 74109 | 0.66 | 0.962698 |
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Target: 5'- gCGGucgacGCUGUCGcgGCuGGGGUccucAUGCGg -3' miRNA: 3'- aGCCu----CGGCAGCuaCG-CCUCA----UAUGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 3403 | 0.66 | 0.974649 |
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Target: 5'- gCGGGGUCGgugCGcgGgGGAGgg-ACGu -3' miRNA: 3'- aGCCUCGGCa--GCuaCgCCUCauaUGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 122430 | 0.66 | 0.974649 |
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Target: 5'- gCGGGGUCGgugCGcgGgGGAGgg-ACGu -3' miRNA: 3'- aGCCUCGGCa--GCuaCgCCUCauaUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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