Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24394 | 5' | -54 | NC_005264.1 | + | 40801 | 0.69 | 0.898981 |
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Target: 5'- gCGGAGCCGcUUGccGCGGGGUcgcCGa -3' miRNA: 3'- aGCCUCGGC-AGCuaCGCCUCAuauGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 149591 | 0.7 | 0.848563 |
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Target: 5'- -gGGGGCUGcUCGAUGCcGAGUcuuUGCGg -3' miRNA: 3'- agCCUCGGC-AGCUACGcCUCAu--AUGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 117421 | 0.7 | 0.832311 |
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Target: 5'- aUCGGGGCUGUCGcgGCGcGcAGg--GCGc -3' miRNA: 3'- -AGCCUCGGCAGCuaCGC-C-UCauaUGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 45914 | 0.72 | 0.751146 |
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Target: 5'- aCGGAGCCGagcagcuaguuUCGAUGCGGuGcaugACGu -3' miRNA: 3'- aGCCUCGGC-----------AGCUACGCCuCaua-UGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 108149 | 0.73 | 0.721893 |
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Target: 5'- -gGGGGCCGUCGAgccucGCGGGGa--GCGu -3' miRNA: 3'- agCCUCGGCAGCUa----CGCCUCauaUGC- -5' |
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| 24394 | 5' | -54 | NC_005264.1 | + | 117112 | 1.1 | 0.004434 |
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Target: 5'- uUCGGAGCCGUCGAUGCGGAGUAUACGg -3' miRNA: 3'- -AGCCUCGGCAGCUACGCCUCAUAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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