Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24396 | 5' | -59.4 | NC_005264.1 | + | 106 | 0.67 | 0.706237 |
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Target: 5'- --cGGGccgCUCGAGuuuccuuuuCCCGCUcCCCGGc -3' miRNA: 3'- aaaCCCaa-GAGCUC---------GGGCGGuGGGCC- -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 118357 | 0.67 | 0.754053 |
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Target: 5'- aUUGGGUcUCgacgUCGAuggucacgaacGCCUGCCGCuuGGu -3' miRNA: 3'- aAACCCA-AG----AGCU-----------CGGGCGGUGggCC- -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 44882 | 0.66 | 0.763345 |
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Target: 5'- -cUGGGgaggCGGGCCCGUCGucgUCUGGg -3' miRNA: 3'- aaACCCaagaGCUCGGGCGGU---GGGCC- -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 119438 | 0.66 | 0.772526 |
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Target: 5'- --cGGGcgaUCUCGcuGCCCagGCCGCCCa- -3' miRNA: 3'- aaaCCCa--AGAGCu-CGGG--CGGUGGGcc -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 30838 | 0.66 | 0.787857 |
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Target: 5'- --cGGGcUCgCGucgucgucggcggcGGCgCGCCGCCCGGu -3' miRNA: 3'- aaaCCCaAGaGC--------------UCGgGCGGUGGGCC- -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 69406 | 0.66 | 0.799324 |
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Target: 5'- --cGGGUaggCGcGCCUGCCGCCguCGGa -3' miRNA: 3'- aaaCCCAagaGCuCGGGCGGUGG--GCC- -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 109975 | 0.66 | 0.799324 |
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Target: 5'- ---aGGUaCcgCGAGCCUGcCCGCUCGGg -3' miRNA: 3'- aaacCCAaGa-GCUCGGGC-GGUGGGCC- -5' |
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| 24396 | 5' | -59.4 | NC_005264.1 | + | 115870 | 1.08 | 0.001695 |
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Target: 5'- gUUUGGGUUCUCGAGCCCGCCACCCGGg -3' miRNA: 3'- -AAACCCAAGAGCUCGGGCGGUGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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