Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24470 | 3' | -57.6 | NC_005264.1 | + | 93824 | 0.68 | 0.740568 |
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Target: 5'- uGGCCgagGUgGCGGuUCGGuugGCcgUGCGCGCa -3' miRNA: 3'- -UCGGa--CAgCGCCuAGUC---UG--ACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 94405 | 0.7 | 0.610046 |
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Target: 5'- cGCagacCGCGGcgCGGACUGCGCGa -3' miRNA: 3'- uCGgacaGCGCCuaGUCUGACGCGUg -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 113365 | 0.67 | 0.775677 |
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Target: 5'- uGCCcGUcgaauacggCGCGGGUCAGgguuaugcaggcgaGCUGCGCGa -3' miRNA: 3'- uCGGaCA---------GCGCCUAGUC--------------UGACGCGUg -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 119478 | 0.7 | 0.630385 |
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Target: 5'- cGGCauauaCUGcUUGUGGAUguGGCUGCGCAg -3' miRNA: 3'- -UCG-----GAC-AGCGCCUAguCUGACGCGUg -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 126615 | 0.66 | 0.85531 |
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Target: 5'- cGCCccuaaaccUCGCGGGcUCuGGuCUGCGCGCa -3' miRNA: 3'- uCGGac------AGCGCCU-AGuCU-GACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 146430 | 0.7 | 0.64056 |
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Target: 5'- uGGCUcgaUGcCGCGGcUCuGACuUGCGCACa -3' miRNA: 3'- -UCGG---ACaGCGCCuAGuCUG-ACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 159775 | 0.67 | 0.80554 |
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Target: 5'- cGCCaGUCGCGGcg-AGACaGCGUGCc -3' miRNA: 3'- uCGGaCAGCGCCuagUCUGaCGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 161640 | 0.66 | 0.85531 |
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Target: 5'- cAGCCaugGcCGCGGAcgccCGGuccGCUGCGUACc -3' miRNA: 3'- -UCGGa--CaGCGCCUa---GUC---UGACGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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