Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24470 | 3' | -57.6 | NC_005264.1 | + | 48096 | 0.66 | 0.847474 |
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Target: 5'- cGGCCg--CGCGGGUUcuGCacGCGCGCg -3' miRNA: 3'- -UCGGacaGCGCCUAGucUGa-CGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 75406 | 0.66 | 0.847474 |
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Target: 5'- cGUCUG-CGCGGccgCGGGCaGUGUACg -3' miRNA: 3'- uCGGACaGCGCCua-GUCUGaCGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 64682 | 0.66 | 0.847474 |
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Target: 5'- gGGCCUGcCgGCGGG-CAGGCcGUGCuCg -3' miRNA: 3'- -UCGGACaG-CGCCUaGUCUGaCGCGuG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 7588 | 0.66 | 0.85531 |
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Target: 5'- cGCCccuaaaccUCGCGGGcUCuGGuCUGCGCGCa -3' miRNA: 3'- uCGGac------AGCGCCU-AGuCU-GACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 42613 | 0.66 | 0.85531 |
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Target: 5'- cAGCCaugGcCGCGGAcgccCGGuccGCUGCGUACc -3' miRNA: 3'- -UCGGa--CaGCGCCUa---GUC---UGACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 126615 | 0.66 | 0.85531 |
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Target: 5'- cGCCccuaaaccUCGCGGGcUCuGGuCUGCGCGCa -3' miRNA: 3'- uCGGac------AGCGCCU-AGuCU-GACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 32541 | 0.66 | 0.85531 |
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Target: 5'- cGGcCCUGgcuGUGGAUguGGCUGC-CGCg -3' miRNA: 3'- -UC-GGACag-CGCCUAguCUGACGcGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 161640 | 0.66 | 0.85531 |
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Target: 5'- cAGCCaugGcCGCGGAcgccCGGuccGCUGCGUACc -3' miRNA: 3'- -UCGGa--CaGCGCCUa---GUC---UGACGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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