Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24470 | 3' | -57.6 | NC_005264.1 | + | 146430 | 0.7 | 0.64056 |
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Target: 5'- uGGCUcgaUGcCGCGGcUCuGACuUGCGCACa -3' miRNA: 3'- -UCGG---ACaGCGCCuAGuCUG-ACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 119478 | 0.7 | 0.630385 |
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Target: 5'- cGGCauauaCUGcUUGUGGAUguGGCUGCGCAg -3' miRNA: 3'- -UCG-----GAC-AGCGCCUAguCUGACGCGUg -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 94405 | 0.7 | 0.610046 |
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Target: 5'- cGCagacCGCGGcgCGGACUGCGCGa -3' miRNA: 3'- uCGgacaGCGCCuaGUCUGACGCGUg -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 20268 | 0.71 | 0.549641 |
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Target: 5'- cGGCC--UCGCGGAggGGACcgucUGCGCGCu -3' miRNA: 3'- -UCGGacAGCGCCUagUCUG----ACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 76345 | 0.71 | 0.549641 |
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Target: 5'- uGGCCg--CGCGGc---GACUGCGCGCa -3' miRNA: 3'- -UCGGacaGCGCCuaguCUGACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 63089 | 0.72 | 0.49121 |
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Target: 5'- gGGCC-GUCGCGGAa-AGGCcuaccucgUGCGCACg -3' miRNA: 3'- -UCGGaCAGCGCCUagUCUG--------ACGCGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 91577 | 0.79 | 0.217701 |
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Target: 5'- aGGCUUGUCGCGGAgcucauaauuguuaUAGGCUGCuGCACg -3' miRNA: 3'- -UCGGACAGCGCCUa-------------GUCUGACG-CGUG- -5' |
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| 24470 | 3' | -57.6 | NC_005264.1 | + | 59346 | 1.1 | 0.001716 |
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Target: 5'- cAGCCUGUCGCGGAUCAGACUGCGCACg -3' miRNA: 3'- -UCGGACAGCGCCUAGUCUGACGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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