miRNA: 3'- uUAGCGUa--CGGCGGCAGcG-UGCUcau -5'
miRNA: 3'- uuaGCGUa-CGG-CGGCAGCGUgCUCau -5'
miRNA: 3'- uUAGCgUaCGGCGGCAGCG------UGCUCAU- -5'
miRNA: 3'- uuaGCGUACGGCG-GcAGCG--UGCUCau -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24480 | 3' | -57.1 | NC_005264.1 | + | 3648 | 0.66 | 0.852118 |
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Target: 5'- cGUCGCGaugGCCGCCGUCuCgACGAu-- -3' miRNA: 3'- uUAGCGUa--CGGCGGCAGcG-UGCUcau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 116370 | 0.66 | 0.852118 |
|
Target: 5'- --cUGUAggGCCuGCCGUCGCGgGAGa- -3' miRNA: 3'- uuaGCGUa-CGG-CGGCAGCGUgCUCau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 27469 | 0.66 | 0.859914 |
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Target: 5'- cAUUGgAgGUCGCCGUCGUacaugaACGAGUAc -3' miRNA: 3'- uUAGCgUaCGGCGGCAGCG------UGCUCAU- -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 123038 | 0.66 | 0.859914 |
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Target: 5'- --aUGCGUGCCGCgCaUCGCcaACGAGa- -3' miRNA: 3'- uuaGCGUACGGCG-GcAGCG--UGCUCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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