miRNA: 3'- uUAGCGuaCGGCGGCAGCGuGCUCau -5'
miRNA: 3'- uUAGCGuaCGGCGGCAGCGuGCUCau -5'
miRNA: 3'- uuaGCGU-ACGGCGGCAGCGUGcucau -5'
miRNA: 3'- -UUAGCGUACGGCGGCAGCGUGCUCAU- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24480 | 3' | -57.1 | NC_005264.1 | + | 132233 | 0.74 | 0.41062 |
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Target: 5'- cGUCGCc-GCCGCCGUCGC-CGGGc- -3' miRNA: 3'- uUAGCGuaCGGCGGCAGCGuGCUCau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 13206 | 0.74 | 0.41062 |
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Target: 5'- cGUCGCc-GCCGCCGUCGC-CGGGc- -3' miRNA: 3'- uUAGCGuaCGGCGGCAGCGuGCUCau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 56322 | 0.74 | 0.385028 |
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Target: 5'- --cCGCGcUGCCGCCGUCGCGCa---- -3' miRNA: 3'- uuaGCGU-ACGGCGGCAGCGUGcucau -5' |
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| 24480 | 3' | -57.1 | NC_005264.1 | + | 49092 | 1.04 | 0.004318 |
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Target: 5'- aAAUCGCAUGCCGCCGUCGCACGAGUAu -3' miRNA: 3'- -UUAGCGUACGGCGGCAGCGUGCUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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