Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24548 | 5' | -59.7 | NC_005264.1 | + | 42731 | 0.71 | 0.456003 |
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Target: 5'- -cUGCGCCccacUUCUGCGCGGCacCCGg-- -3' miRNA: 3'- acACGCGG----AAGACGCGCCGc-GGCaag -5' |
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| 24548 | 5' | -59.7 | NC_005264.1 | + | 61454 | 0.72 | 0.403672 |
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Target: 5'- --gGCGCCgagUUGCuCGaGCGCCGUUCg -3' miRNA: 3'- acaCGCGGaa-GACGcGC-CGCGGCAAG- -5' |
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| 24548 | 5' | -59.7 | NC_005264.1 | + | 66608 | 0.72 | 0.395325 |
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Target: 5'- -cUGCGCCgcgUUGC-CGGCGCCGUc- -3' miRNA: 3'- acACGCGGaa-GACGcGCCGCGGCAag -5' |
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| 24548 | 5' | -59.7 | NC_005264.1 | + | 46454 | 0.74 | 0.304222 |
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Target: 5'- -cUGCGgCUUUUGCGCGGCGCUcUUUg -3' miRNA: 3'- acACGCgGAAGACGCGCCGCGGcAAG- -5' |
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| 24548 | 5' | -59.7 | NC_005264.1 | + | 136667 | 0.9 | 0.028346 |
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Target: 5'- uUGUGCGCCgcggcaggCUGCGCGGCGCCGUcUCu -3' miRNA: 3'- -ACACGCGGaa------GACGCGCCGCGGCA-AG- -5' |
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| 24548 | 5' | -59.7 | NC_005264.1 | + | 4768 | 1.1 | 0.001122 |
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Target: 5'- aUGUGCGCCUUCUGCGCGGCGCCGUUCg -3' miRNA: 3'- -ACACGCGGAAGACGCGCCGCGGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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