miRNA: 3'- gCUaCUGC--AGACGGCGCCGAgaUUGa -5'
miRNA: 3'- gcuAC-UGCAG----ACGGcGCCGAGAUUGa -5'
miRNA: 3'- gCUACUGCaGACGG---CGCCGa-GAuUGa -5'
miRNA: 3'- gCUACUGcAG--ACGGCGCCGAGAUUGA- -5'
miRNA: 3'- -GCUACUGCA-GAcGGCGCCGa-------Ga--UUGa -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24612 | 5' | -54.5 | NC_005264.1 | + | 98766 | 0.68 | 0.895667 |
|
Target: 5'- gGAaGGCGgcUCUGCUGCGGCU--GACa -3' miRNA: 3'- gCUaCUGC--AGACGGCGCCGAgaUUGa -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 107721 | 0.67 | 0.914374 |
|
Target: 5'- --cUGaACGUCcgagUGCCcCGGCUCUGGCa -3' miRNA: 3'- gcuAC-UGCAG----ACGGcGCCGAGAUUGa -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 127001 | 0.67 | 0.930931 |
|
Target: 5'- aGAUGACG-CUGCCcacGCGGCaaCUcGCa -3' miRNA: 3'- gCUACUGCaGACGG---CGCCGa-GAuUGa -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 146427 | 0.84 | 0.171421 |
|
Target: 5'- uGAUGGC-UCgaUGCCGCGGCUCUGACUu -3' miRNA: 3'- gCUACUGcAG--ACGGCGCCGAGAUUGA- -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 157822 | 0.67 | 0.930931 |
|
Target: 5'- cCGGUGACGUaCU-CCGCGGCgaccccggCgcgGACg -3' miRNA: 3'- -GCUACUGCA-GAcGGCGCCGa-------Ga--UUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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