miRNA: 3'- gCUAcUGC-AGACGgCGCCG---AGAUUGa -5'
miRNA: 3'- -GCuaCugCAGaCGGCGCCGa-Ga---UUGa -5'
miRNA: 3'- gcuaCUGcAGACGGCGC-CGAGa---UUGA- -5'
miRNA: 3'- -GCUACUGCaGa--CGGCG-CCGa-GAUUGa -5'
miRNA: 3'- gcuaCUGCAGaCGG-CGCCGaGAUUGa -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24612 | 5' | -54.5 | NC_005264.1 | + | 2646 | 0.66 | 0.953733 |
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Target: 5'- aGGUuGCGcUCUGCaGCGGCagcUCUGACg -3' miRNA: 3'- gCUAcUGC-AGACGgCGCCG---AGAUUGa -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 91888 | 0.66 | 0.957591 |
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Target: 5'- gCGggGugGUUgGCCGCGGCagCgaugGGCg -3' miRNA: 3'- -GCuaCugCAGaCGGCGCCGa-Ga---UUGa -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 63812 | 0.66 | 0.961224 |
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Target: 5'- ---cGAUuUCUGCCGCGcGCUCgaccAGCUc -3' miRNA: 3'- gcuaCUGcAGACGGCGC-CGAGa---UUGA- -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 93837 | 0.66 | 0.961224 |
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Target: 5'- aCGAUGGCGaCaggGCCGCgGGCcaCUGGCc -3' miRNA: 3'- -GCUACUGCaGa--CGGCG-CCGa-GAUUGa -5' |
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| 24612 | 5' | -54.5 | NC_005264.1 | + | 88157 | 0.66 | 0.961224 |
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Target: 5'- ---aGACGUC-GCCgGUGGCgCUAACg -3' miRNA: 3'- gcuaCUGCAGaCGG-CGCCGaGAUUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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