Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 247 | 3' | -57.6 | AC_000008.1 | + | 4607 | 0.66 | 0.396405 |
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Target: 5'- --aUGGCAAuGGGCccacGGGCGGCGGCc -3' miRNA: 3'- gucGCCGUU-CUCGuu--CUCGUCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 17274 | 0.66 | 0.41562 |
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Target: 5'- uGGCGGCGcGGcGCAGGGacGC-GCGGCu -3' miRNA: 3'- gUCGCCGUuCU-CGUUCU--CGuCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 11792 | 0.66 | 0.41562 |
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Target: 5'- uGGCGGCAucGGuGgAGGcGGUGGUGGCGa -3' miRNA: 3'- gUCGCCGU--UCuCgUUC-UCGUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 12290 | 0.66 | 0.425439 |
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Target: 5'- aGGaGGUAAGcGCAcAGuGCGGCGGCu -3' miRNA: 3'- gUCgCCGUUCuCGU-UCuCGUCGCCGu -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 24602 | 0.66 | 0.425439 |
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Target: 5'- gAGCGGaCAAGcAGCu--GGCcuuGCGGCAg -3' miRNA: 3'- gUCGCC-GUUC-UCGuucUCGu--CGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 14192 | 0.66 | 0.425439 |
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Target: 5'- -uGUGGUGAGcgcGGCGccAGuGGCGGCGGCGc -3' miRNA: 3'- guCGCCGUUC---UCGU--UC-UCGUCGCCGU- -5' |
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| 247 | 3' | -57.6 | AC_000008.1 | + | 8611 | 0.66 | 0.425439 |
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Target: 5'- gGGgGGCuccggacccgccGGGAGaGGGGGCAGgGGCAc -3' miRNA: 3'- gUCgCCG------------UUCUCgUUCUCGUCgCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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