Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24807 | 3' | -56.6 | NC_005284.1 | + | 915 | 0.66 | 0.698561 |
Target: 5'- gGGCGUugGCaUCGGCAUguucgugcuggacgaCGAGUUCGg -3' miRNA: 3'- gCCGCAugUGgAGCCGUG---------------GCUUGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 5912 | 0.66 | 0.683662 |
Target: 5'- gGGCGUgu-CCaCGGCGCCGAcuuCUCc -3' miRNA: 3'- gCCGCAuguGGaGCCGUGGCUu--GAGc -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 44959 | 0.66 | 0.683662 |
Target: 5'- gGGCGUGC-CCgugaugCGcGCGCCGAuUUCc -3' miRNA: 3'- gCCGCAUGuGGa-----GC-CGUGGCUuGAGc -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 34845 | 0.66 | 0.683662 |
Target: 5'- uGGCGUcGCACa--GGCGCaCGAuugggaucgGCUCGa -3' miRNA: 3'- gCCGCA-UGUGgagCCGUG-GCU---------UGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 54322 | 0.66 | 0.672961 |
Target: 5'- uCGGCuuUGCGCCgaccaGGCGCUGccGGCUCGc -3' miRNA: 3'- -GCCGc-AUGUGGag---CCGUGGC--UUGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 21613 | 0.66 | 0.672961 |
Target: 5'- gGGCa-AgAUCUCGcGCGCCGcGCUCGa -3' miRNA: 3'- gCCGcaUgUGGAGC-CGUGGCuUGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 4482 | 0.66 | 0.672961 |
Target: 5'- uGGCucGCGgCggCGGUagcGCCGAACUCGa -3' miRNA: 3'- gCCGcaUGUgGa-GCCG---UGGCUUGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 10589 | 0.66 | 0.651459 |
Target: 5'- gCGcGCGUugACgCUCuggacGCACCGGACUUc -3' miRNA: 3'- -GC-CGCAugUG-GAGc----CGUGGCUUGAGc -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 38720 | 0.66 | 0.651459 |
Target: 5'- cCGGCucaaUGCGCUgcaUGGCGCCGGacggcaugaGCUCGa -3' miRNA: 3'- -GCCGc---AUGUGGa--GCCGUGGCU---------UGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 49089 | 0.66 | 0.651459 |
Target: 5'- gCGGUaaGCAaCUCGGCAUCGAGCagcgCGa -3' miRNA: 3'- -GCCGcaUGUgGAGCCGUGGCUUGa---GC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 31432 | 0.66 | 0.651459 |
Target: 5'- gGGCGacgUGCGCCgcugCGGCgcGCCGGGCc-- -3' miRNA: 3'- gCCGC---AUGUGGa---GCCG--UGGCUUGagc -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 6622 | 0.66 | 0.650381 |
Target: 5'- gGGCGU-CACUUCGccagcgcGCGCCGGaauggGCUCu -3' miRNA: 3'- gCCGCAuGUGGAGC-------CGUGGCU-----UGAGc -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 47074 | 0.66 | 0.640677 |
Target: 5'- gCGGCGgcacgaGCUUCGGCGCaccGCUCa -3' miRNA: 3'- -GCCGCaug---UGGAGCCGUGgcuUGAGc -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 48761 | 0.67 | 0.6191 |
Target: 5'- gCGGCGUGCGCCgcgaguugCGuuGCAUggCGAACUgCGa -3' miRNA: 3'- -GCCGCAUGUGGa-------GC--CGUG--GCUUGA-GC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 11892 | 0.67 | 0.6191 |
Target: 5'- gGGUGcGCGCCucUCGGCAUCGuccaUCGa -3' miRNA: 3'- gCCGCaUGUGG--AGCCGUGGCuug-AGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 40162 | 0.67 | 0.6191 |
Target: 5'- aCGGCGUcgagguagcGCGCgagcgUUCGGCGCCGGAacacuUUCGc -3' miRNA: 3'- -GCCGCA---------UGUG-----GAGCCGUGGCUU-----GAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 16205 | 0.67 | 0.6191 |
Target: 5'- -cGCGUGCG-CUCGGCcgaacgGCCGucucGCUCGg -3' miRNA: 3'- gcCGCAUGUgGAGCCG------UGGCu---UGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 16081 | 0.68 | 0.533896 |
Target: 5'- gGGCucuucaACACgauuCUCGGCGCCGcACUCGu -3' miRNA: 3'- gCCGca----UGUG----GAGCCGUGGCuUGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 47806 | 0.68 | 0.523497 |
Target: 5'- gGGCGaGCGCUuggCGGCGCUGcgauggguGCUCGg -3' miRNA: 3'- gCCGCaUGUGGa--GCCGUGGCu-------UGAGC- -5' |
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24807 | 3' | -56.6 | NC_005284.1 | + | 16762 | 0.69 | 0.502942 |
Target: 5'- uGcGCGUGCGCUUCGGUguGCCGGcGCUa- -3' miRNA: 3'- gC-CGCAUGUGGAGCCG--UGGCU-UGAgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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