Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24816 | 5' | -57.5 | NC_005284.1 | + | 1817 | 0.66 | 0.646813 |
Target: 5'- gCCUCGGCaaCUCGaaGcAUCGGCaggauuGCGUCGa -3' miRNA: 3'- -GGAGCCG--GAGCggC-UAGCUG------CGUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 18918 | 0.66 | 0.640382 |
Target: 5'- gCUUGGCacggagcugcgcgCGCCGAUCGACcuaAUCAc -3' miRNA: 3'- gGAGCCGga-----------GCGGCUAGCUGcg-UAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 7725 | 0.66 | 0.636094 |
Target: 5'- gCUCGuGCCgcCGCCGG-CGGCGUAa-- -3' miRNA: 3'- gGAGC-CGGa-GCGGCUaGCUGCGUagu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 32797 | 0.66 | 0.625372 |
Target: 5'- gCgUCGGCCUgCGCgGGUUgcggGGCgGCGUCGg -3' miRNA: 3'- -GgAGCCGGA-GCGgCUAG----CUG-CGUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 41609 | 0.66 | 0.625372 |
Target: 5'- ---aGGCCgCGCCGAgugCGAgcuccgacaCGCAUCGg -3' miRNA: 3'- ggagCCGGaGCGGCUa--GCU---------GCGUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 45150 | 0.66 | 0.624299 |
Target: 5'- gCUCGGCCUgcgcugaagccucCGCCGcUUG-CGCGUUc -3' miRNA: 3'- gGAGCCGGA-------------GCGGCuAGCuGCGUAGu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 32148 | 0.66 | 0.614655 |
Target: 5'- --gCGGCCgaUCGUaaCGAUCGAUGCAggUCGc -3' miRNA: 3'- ggaGCCGG--AGCG--GCUAGCUGCGU--AGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 1167 | 0.66 | 0.603953 |
Target: 5'- gCUC-GUCUCGUCGAUCuGCGUAUUg -3' miRNA: 3'- gGAGcCGGAGCGGCUAGcUGCGUAGu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 42995 | 0.66 | 0.603953 |
Target: 5'- gCUCcGCCUCgGCCGuGUUGAUGCGcUCGa -3' miRNA: 3'- gGAGcCGGAG-CGGC-UAGCUGCGU-AGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 31141 | 0.66 | 0.603953 |
Target: 5'- -gUCGGCCUCuucCUGGUUGACGauCGUCGc -3' miRNA: 3'- ggAGCCGGAGc--GGCUAGCUGC--GUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 46533 | 0.66 | 0.593274 |
Target: 5'- gCUCGGCCaacgucUUGCCGAgccgcagcgCGAgCGaCAUCAg -3' miRNA: 3'- gGAGCCGG------AGCGGCUa--------GCU-GC-GUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 52047 | 0.67 | 0.582625 |
Target: 5'- uCCUCGGCCagcgUGCUGAcaguuccaUCGACGUu--- -3' miRNA: 3'- -GGAGCCGGa---GCGGCU--------AGCUGCGuagu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 5624 | 0.67 | 0.562508 |
Target: 5'- uCCUCcaucGCCUCGCCGGgcccguccgccuugaCGACGCGc-- -3' miRNA: 3'- -GGAGc---CGGAGCGGCUa--------------GCUGCGUagu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 21207 | 0.67 | 0.540498 |
Target: 5'- -aUCGGCUUCGCUGGUgGGUGCcgaaGUCAg -3' miRNA: 3'- ggAGCCGGAGCGGCUAgCUGCG----UAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 42191 | 0.68 | 0.530119 |
Target: 5'- aCUCGGUCUCGgcCCGGUCGGgauuuauaucCGCAg-- -3' miRNA: 3'- gGAGCCGGAGC--GGCUAGCU----------GCGUagu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 15172 | 0.68 | 0.530119 |
Target: 5'- aCCUugCGGCCgUGCCGAcCGAccaguucgcgcuCGCGUCGg -3' miRNA: 3'- -GGA--GCCGGaGCGGCUaGCU------------GCGUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 34587 | 0.68 | 0.530119 |
Target: 5'- uUCUCGGCgUCGCUGuaUGGCGaAUCAg -3' miRNA: 3'- -GGAGCCGgAGCGGCuaGCUGCgUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 453 | 0.68 | 0.519812 |
Target: 5'- cCCggCGaGCCggcagCGCCuGGUCGGCGCAa-- -3' miRNA: 3'- -GGa-GC-CGGa----GCGG-CUAGCUGCGUagu -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 11768 | 0.68 | 0.509586 |
Target: 5'- gCC-CGaGUacaUCGCCGAgaUCGAgCGCAUCAa -3' miRNA: 3'- -GGaGC-CGg--AGCGGCU--AGCU-GCGUAGU- -5' |
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24816 | 5' | -57.5 | NC_005284.1 | + | 48125 | 0.68 | 0.509586 |
Target: 5'- --cCGGCCgUCGCCaaaccCGGCGCGUCu -3' miRNA: 3'- ggaGCCGG-AGCGGcua--GCUGCGUAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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