Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24817 | 5' | -57.4 | NC_005284.1 | + | 48270 | 0.66 | 0.606331 |
Target: 5'- cGUGCgGGCACUGAGGaGCCUaugaGCGu -3' miRNA: 3'- -CACG-CCGUGGCUCUaUGGGgaagCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 47240 | 0.66 | 0.595443 |
Target: 5'- -cGCGGCACgcAGAauguuUugCCCUUCGUc -3' miRNA: 3'- caCGCCGUGgcUCU-----AugGGGAAGCGu -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 41365 | 0.66 | 0.572687 |
Target: 5'- cUGCGGauCGCCGAGAcACgCCCgcggucacgauguUUCGCGa -3' miRNA: 3'- cACGCC--GUGGCUCUaUG-GGG-------------AAGCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 16198 | 0.66 | 0.552278 |
Target: 5'- -gGCGGCAUCGugcAGAUGCUCagucCGCAa -3' miRNA: 3'- caCGCCGUGGC---UCUAUGGGgaa-GCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 7117 | 0.67 | 0.541624 |
Target: 5'- -aGCGGCAUCGAucaacGCCugaaCCUUCGCGc -3' miRNA: 3'- caCGCCGUGGCUcua--UGG----GGAAGCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 8982 | 0.68 | 0.478367 |
Target: 5'- cGUGCGGacaccuuCGCCGAGcAUGCCgacgCCUgCGCAa -3' miRNA: 3'- -CACGCC-------GUGGCUC-UAUGG----GGAaGCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 30424 | 0.68 | 0.430298 |
Target: 5'- cGUGCGcGaCGgCGAGGUGCUgUUUCGCGa -3' miRNA: 3'- -CACGC-C-GUgGCUCUAUGGgGAAGCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 38493 | 0.69 | 0.402304 |
Target: 5'- -aGCGGCACCGGcucGCCCUg-CGCAg -3' miRNA: 3'- caCGCCGUGGCUcuaUGGGGaaGCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 48928 | 0.7 | 0.336794 |
Target: 5'- uUGCGGCGCCGAuguagaucuugaucaGAUcgucggcaACCCCUgcguccUCGCGc -3' miRNA: 3'- cACGCCGUGGCU---------------CUA--------UGGGGA------AGCGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 33178 | 0.7 | 0.333569 |
Target: 5'- -cGCGGCGCgCGAGAUcuuGCCCaacgCUUCgGCAu -3' miRNA: 3'- caCGCCGUG-GCUCUA---UGGG----GAAG-CGU- -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 38700 | 0.77 | 0.118309 |
Target: 5'- aGUGCGGCGCCGAGAaucguguugaagaGCCCCccgCGCu -3' miRNA: 3'- -CACGCCGUGGCUCUa------------UGGGGaa-GCGu -5' |
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24817 | 5' | -57.4 | NC_005284.1 | + | 47860 | 0.95 | 0.006514 |
Target: 5'- gGUGCGaCGCCGAGAUACCCCUUCGCAc -3' miRNA: 3'- -CACGCcGUGGCUCUAUGGGGAAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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