Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24820 | 3' | -54.2 | NC_005284.1 | + | 34048 | 0.66 | 0.81392 |
Target: 5'- aGGAU--CGUCgcugGCUU-CGCGGCUCGGa -3' miRNA: 3'- -CCUGcuGUAGa---CGAGcGUGUCGAGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 5281 | 0.66 | 0.81392 |
Target: 5'- uGAUGcCGUCgUGCUCGCACuucguGUUgCGGu -3' miRNA: 3'- cCUGCuGUAG-ACGAGCGUGu----CGA-GCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 16196 | 0.66 | 0.812985 |
Target: 5'- uGGGCGGCAUCgugcagaUGCUCaguccGCAacaGGC-CGGc -3' miRNA: 3'- -CCUGCUGUAG-------ACGAG-----CGUg--UCGaGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 12645 | 0.66 | 0.804486 |
Target: 5'- -uACGACG-CUGCgugugCGCAaagccCAGUUCGGc -3' miRNA: 3'- ccUGCUGUaGACGa----GCGU-----GUCGAGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 3457 | 0.66 | 0.804486 |
Target: 5'- --cCGAgcCAUUUGCgcuguuccUCGgACAGCUCGGg -3' miRNA: 3'- ccuGCU--GUAGACG--------AGCgUGUCGAGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 13592 | 0.66 | 0.794872 |
Target: 5'- gGGAagaaGACg---GCUgGC-CAGCUCGGg -3' miRNA: 3'- -CCUg---CUGuagaCGAgCGuGUCGAGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 32981 | 0.66 | 0.794872 |
Target: 5'- aGGGCGGCGgucaCUcGCUC-CGCAGCgggCGa -3' miRNA: 3'- -CCUGCUGUa---GA-CGAGcGUGUCGa--GCc -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 45151 | 0.66 | 0.794872 |
Target: 5'- aGGCGugGUCga--CGCACGGCgacgUCGGg -3' miRNA: 3'- cCUGCugUAGacgaGCGUGUCG----AGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 9415 | 0.66 | 0.78509 |
Target: 5'- cGACGGCAUCgugaaacucgGCgugUCGCucGCGGC-CGGg -3' miRNA: 3'- cCUGCUGUAGa---------CG---AGCG--UGUCGaGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 13101 | 0.66 | 0.78509 |
Target: 5'- cGGACGAUAUCgGaaa--GCGGUUCGGg -3' miRNA: 3'- -CCUGCUGUAGaCgagcgUGUCGAGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 38744 | 0.66 | 0.775149 |
Target: 5'- cGGACGGCAUgaGCUCGauu-GCgUCGa -3' miRNA: 3'- -CCUGCUGUAgaCGAGCguguCG-AGCc -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 45459 | 0.66 | 0.775149 |
Target: 5'- cGGGCGACcagUUGCUCGCggcaaugacaACGGuCUCa- -3' miRNA: 3'- -CCUGCUGua-GACGAGCG----------UGUC-GAGcc -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 48960 | 0.66 | 0.765061 |
Target: 5'- cGGACuGCAUCgacgaagcggUGCU-GCGCGGCUggaCGGg -3' miRNA: 3'- -CCUGcUGUAG----------ACGAgCGUGUCGA---GCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 33968 | 0.66 | 0.765061 |
Target: 5'- cGGcCGGCG-CUGCUgCGgGCuGCUCGa -3' miRNA: 3'- -CCuGCUGUaGACGA-GCgUGuCGAGCc -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 23030 | 0.66 | 0.764044 |
Target: 5'- uGACGACAagcUCUGCgcagaGCGCuaucgcuGGCUgCGGg -3' miRNA: 3'- cCUGCUGU---AGACGag---CGUG-------UCGA-GCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 44762 | 0.67 | 0.754836 |
Target: 5'- -cGCGACAUCacgcucgGCUUGCGCAagGCcgCGGu -3' miRNA: 3'- ccUGCUGUAGa------CGAGCGUGU--CGa-GCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 45848 | 0.67 | 0.754836 |
Target: 5'- -uGCGACGcgUUGUUUGCgauccgcugauACAGCUCGGc -3' miRNA: 3'- ccUGCUGUa-GACGAGCG-----------UGUCGAGCC- -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 13377 | 0.67 | 0.754836 |
Target: 5'- uGGGCGGCgAUCUGUcUGCgGCGGUUCa- -3' miRNA: 3'- -CCUGCUG-UAGACGaGCG-UGUCGAGcc -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 46897 | 0.67 | 0.744488 |
Target: 5'- cGGucgcCGACGUCgGCUCGCGCA-CUgCGa -3' miRNA: 3'- -CCu---GCUGUAGaCGAGCGUGUcGA-GCc -5' |
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24820 | 3' | -54.2 | NC_005284.1 | + | 31431 | 0.67 | 0.744488 |
Target: 5'- cGGGCGACGUgcGC-CGCuGCGGCgcgcCGGg -3' miRNA: 3'- -CCUGCUGUAgaCGaGCG-UGUCGa---GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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