Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24822 | 3' | -52.3 | NC_005284.1 | + | 44370 | 0.66 | 0.821307 |
Target: 5'- uAUGCCGACUGCG-CGuCAucGGCc--- -3' miRNA: 3'- uUACGGCUGACGUaGC-GUu-CCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 41050 | 0.66 | 0.821307 |
Target: 5'- --cGCCGAC-GUuucUCGCGGGGCg--- -3' miRNA: 3'- uuaCGGCUGaCGu--AGCGUUCCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 33127 | 0.66 | 0.821307 |
Target: 5'- --cGCCGACgcggcgGCAUCGUgcgcGGCgaUCg -3' miRNA: 3'- uuaCGGCUGa-----CGUAGCGuu--CCGaaAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 10806 | 0.66 | 0.811541 |
Target: 5'- --cGCCGGCggaaugGCAUCG-GAGGCg--- -3' miRNA: 3'- uuaCGGCUGa-----CGUAGCgUUCCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 44663 | 0.66 | 0.801567 |
Target: 5'- --cGCCGuCUGCGccgUCGCcgcGCUUUCg -3' miRNA: 3'- uuaCGGCuGACGU---AGCGuucCGAAAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 31619 | 0.66 | 0.801567 |
Target: 5'- gGGUGCgGGCgGCGUCG-AGGGCgcaUUCc -3' miRNA: 3'- -UUACGgCUGaCGUAGCgUUCCGa--AAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 50168 | 0.66 | 0.791399 |
Target: 5'- -cUGCCGAC--CGUCGCA-GGCacgUUCa -3' miRNA: 3'- uuACGGCUGacGUAGCGUuCCGa--AAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 17713 | 0.67 | 0.759847 |
Target: 5'- --cGCCGGC-GCGUCGau-GGCggUCg -3' miRNA: 3'- uuaCGGCUGaCGUAGCguuCCGaaAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 16216 | 0.67 | 0.727001 |
Target: 5'- --gGCCGAacgGCcgucUCGCucGGGCUUUCg -3' miRNA: 3'- uuaCGGCUga-CGu---AGCGu-UCCGAAAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 48917 | 0.68 | 0.715826 |
Target: 5'- --aGCCGAUUGCGUUGC--GGCg--- -3' miRNA: 3'- uuaCGGCUGACGUAGCGuuCCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 35167 | 0.68 | 0.70456 |
Target: 5'- uAUGCCGugUGCgAUCGCGGcGCa--- -3' miRNA: 3'- uUACGGCugACG-UAGCGUUcCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 49050 | 0.68 | 0.70456 |
Target: 5'- -cUGCCGACUgGCAcaagagcgCGCAGGGUg--- -3' miRNA: 3'- uuACGGCUGA-CGUa-------GCGUUCCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 24344 | 0.68 | 0.70456 |
Target: 5'- --cGCUuAUcGCGUCGCAAGGCUUc- -3' miRNA: 3'- uuaCGGcUGaCGUAGCGUUCCGAAag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 48274 | 0.68 | 0.681809 |
Target: 5'- --cGUCGACUGCGauuUCGCAAG-CUUcUCg -3' miRNA: 3'- uuaCGGCUGACGU---AGCGUUCcGAA-AG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 40770 | 0.68 | 0.681809 |
Target: 5'- --gGCCGACUGUGUCG--AGGCa--- -3' miRNA: 3'- uuaCGGCUGACGUAGCguUCCGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 21558 | 0.69 | 0.658848 |
Target: 5'- -uUGCCGACgagcagGCcgCGC-GGGCgUUCg -3' miRNA: 3'- uuACGGCUGa-----CGuaGCGuUCCGaAAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 50052 | 0.69 | 0.623077 |
Target: 5'- --cGCCGACUGaGUCGCcgcaagaGAGGCUc-- -3' miRNA: 3'- uuaCGGCUGACgUAGCG-------UUCCGAaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 351 | 0.69 | 0.612693 |
Target: 5'- --cGUCGACaGC-UCGCAAGGCagUCc -3' miRNA: 3'- uuaCGGCUGaCGuAGCGUUCCGaaAG- -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 53783 | 0.69 | 0.601173 |
Target: 5'- uGAUGCCGGCUGCugcgGCGAGcGCa--- -3' miRNA: 3'- -UUACGGCUGACGuag-CGUUC-CGaaag -5' |
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24822 | 3' | -52.3 | NC_005284.1 | + | 53498 | 0.7 | 0.566828 |
Target: 5'- --cGCCGGUUcGCcgCGCGGGGCUUUUu -3' miRNA: 3'- uuaCGGCUGA-CGuaGCGUUCCGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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