miRNA: 3'- uuACGGCUG--ACGUAGCGuu--CCGAaAG- -5'
miRNA: 3'- -UUACGGCUGaCGUa-GCGUUCCG---------AAAG- -5'
miRNA: 3'- -UUACGGCUGACGUAGCGUUCCGAAAG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24822 | 3' | -52.3 | NC_005284.1 | + | 49593 | 0.7 | 0.544206 |
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Target: 5'- -uUGCCGACgaUGUAUCGCcccuGGCUgUCc -3' miRNA: 3'- uuACGGCUG--ACGUAGCGuu--CCGAaAG- -5' |
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| 24822 | 3' | -52.3 | NC_005284.1 | + | 43535 | 0.72 | 0.467797 |
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Target: 5'- cGAUGCCGAUaGCGcgCGCAAGGUccggacgggUUUCg -3' miRNA: 3'- -UUACGGCUGaCGUa-GCGUUCCG---------AAAG- -5' |
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| 24822 | 3' | -52.3 | NC_005284.1 | + | 45100 | 1.08 | 0.00157 |
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Target: 5'- cAAUGCCGACUGCAUCGCAAGGCUUUCg -3' miRNA: 3'- -UUACGGCUGACGUAGCGUUCCGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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