Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24822 | 5' | -62.1 | NC_005284.1 | + | 32772 | 0.7 | 0.195737 |
Target: 5'- gUCGC--GCUCGCGcGCcucugccggugcgUCGGCCUGCGc -3' miRNA: 3'- -AGCGuaCGGGCGCuCG-------------AGCCGGACGC- -5' |
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24822 | 5' | -62.1 | NC_005284.1 | + | 30659 | 0.71 | 0.176951 |
Target: 5'- gUGCGUGCggCCGuCGAGCagcCGGCCgGCGg -3' miRNA: 3'- aGCGUACG--GGC-GCUCGa--GCCGGaCGC- -5' |
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24822 | 5' | -62.1 | NC_005284.1 | + | 38559 | 0.71 | 0.176951 |
Target: 5'- uUCGcCGUcCCCGCGAgGC-CGGCCUGUu -3' miRNA: 3'- -AGC-GUAcGGGCGCU-CGaGCCGGACGc -5' |
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24822 | 5' | -62.1 | NC_005284.1 | + | 42912 | 0.71 | 0.172399 |
Target: 5'- cCGCAUcGCCCGCuucacgccGAcGCgccagUCGGCCUGCa -3' miRNA: 3'- aGCGUA-CGGGCG--------CU-CG-----AGCCGGACGc -5' |
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24822 | 5' | -62.1 | NC_005284.1 | + | 39423 | 0.71 | 0.167952 |
Target: 5'- gUCGUcgAUGCCgauCGAGCcaUCGGCCUGCa -3' miRNA: 3'- -AGCG--UACGGgc-GCUCG--AGCCGGACGc -5' |
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24822 | 5' | -62.1 | NC_005284.1 | + | 29691 | 0.73 | 0.12545 |
Target: 5'- aUCGCuuuuUGCCggugacgauCGCGAGgUCGGCCgGCGu -3' miRNA: 3'- -AGCGu---ACGG---------GCGCUCgAGCCGGaCGC- -5' |
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24822 | 5' | -62.1 | NC_005284.1 | + | 45135 | 1.07 | 0.000278 |
Target: 5'- cUCGCAUGCCCGCGAGCUCGGCCUGCGc -3' miRNA: 3'- -AGCGUACGGGCGCUCGAGCCGGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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