Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24823 | 3' | -59.4 | NC_005284.1 | + | 53501 | 0.66 | 0.518044 |
Target: 5'- cGGuUCGCCGCGCGgggcuuuuucauUUCCGCgcccggagUUGCUa-- -3' miRNA: 3'- -CC-AGCGGCGCGC------------AAGGCG--------AGCGAgug -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 31220 | 0.66 | 0.507902 |
Target: 5'- -aUCGCCGCGaCGUUgCCGaugCGCUUc- -3' miRNA: 3'- ccAGCGGCGC-GCAA-GGCga-GCGAGug -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 33441 | 0.66 | 0.497845 |
Target: 5'- --aCGCCuGCGCGguagucgaUCCGCagUGCUUACa -3' miRNA: 3'- ccaGCGG-CGCGCa-------AGGCGa-GCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 15845 | 0.66 | 0.487878 |
Target: 5'- -cUCGgCGUGCGUUUCGgaCGCauUCACc -3' miRNA: 3'- ccAGCgGCGCGCAAGGCgaGCG--AGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 40284 | 0.66 | 0.487878 |
Target: 5'- uGGcUGCUGCGCGUcggaugUCUGCUCGaagccCGCg -3' miRNA: 3'- -CCaGCGGCGCGCA------AGGCGAGCga---GUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 33222 | 0.67 | 0.478006 |
Target: 5'- cGUCGaacgcCCGCGCGgcCUGCUCGUcgGCa -3' miRNA: 3'- cCAGC-----GGCGCGCaaGGCGAGCGagUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 31010 | 0.67 | 0.458564 |
Target: 5'- --cCGCCGUGCGUgcggaaguugCCGgUUgGCUCGCu -3' miRNA: 3'- ccaGCGGCGCGCAa---------GGC-GAgCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 14597 | 0.67 | 0.458564 |
Target: 5'- cGUCGgCGCgaaaGUGUUCCGgCgccgaaCGCUCGCg -3' miRNA: 3'- cCAGCgGCG----CGCAAGGC-Ga-----GCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 3104 | 0.67 | 0.457603 |
Target: 5'- --cUGCCGCugguaucGCGUUCCGC-CGUUUAUg -3' miRNA: 3'- ccaGCGGCG-------CGCAAGGCGaGCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 40700 | 0.67 | 0.448053 |
Target: 5'- -cUCGCCGaGCaGUUCCGggaaaucccaccaCUCGUUCGCg -3' miRNA: 3'- ccAGCGGCgCG-CAAGGC-------------GAGCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 6422 | 0.67 | 0.439553 |
Target: 5'- cGUCGUCGUGCGaauggUCCGCaagUGCcagCGCg -3' miRNA: 3'- cCAGCGGCGCGCa----AGGCGa--GCGa--GUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 6268 | 0.67 | 0.427439 |
Target: 5'- cGGUauUCGCGCGUauUCCcacgcccgcccuucGaCUCGCUCACg -3' miRNA: 3'- -CCAgcGGCGCGCA--AGG--------------C-GAGCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 30250 | 0.68 | 0.420998 |
Target: 5'- aGUCgGCCGUcgaGCGggCgCGUUCGUUCGCu -3' miRNA: 3'- cCAG-CGGCG---CGCaaG-GCGAGCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 15806 | 0.68 | 0.4119 |
Target: 5'- cGUCGCCaGCGCGcagcCCGCggaucagcaUCGCgCACg -3' miRNA: 3'- cCAGCGG-CGCGCaa--GGCG---------AGCGaGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 3532 | 0.68 | 0.394075 |
Target: 5'- -uUCuUCGCGCGUg--GCUCGCUCGCc -3' miRNA: 3'- ccAGcGGCGCGCAaggCGAGCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 41814 | 0.68 | 0.385351 |
Target: 5'- uGUCGCCGCcacUUCCGCUgggugGCUCGCc -3' miRNA: 3'- cCAGCGGCGcgcAAGGCGAg----CGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 23443 | 0.68 | 0.376756 |
Target: 5'- --cCGacagCGCGUGUUCUGCgcgUCGCUCGCc -3' miRNA: 3'- ccaGCg---GCGCGCAAGGCG---AGCGAGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 12362 | 0.69 | 0.368291 |
Target: 5'- -uUCGCCGCGgGUgg-GCUCGUUgGCg -3' miRNA: 3'- ccAGCGGCGCgCAaggCGAGCGAgUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 9638 | 0.69 | 0.355022 |
Target: 5'- cGUCGCCGUGCGUcgaCCacgccuugcccaucaGCUCGacgaUCGCa -3' miRNA: 3'- cCAGCGGCGCGCAa--GG---------------CGAGCg---AGUG- -5' |
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24823 | 3' | -59.4 | NC_005284.1 | + | 28458 | 0.69 | 0.355022 |
Target: 5'- --gUGCCGCGCuGaagaauguccaugcuUUCCGCUCGCaCGCg -3' miRNA: 3'- ccaGCGGCGCG-C---------------AAGGCGAGCGaGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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