Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24824 | 3' | -53.8 | NC_005284.1 | + | 5346 | 0.67 | 0.778457 |
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Target: 5'- -aAGAGUggCCGGUcgAGGUCGCCGGa- -3' miRNA: 3'- ggUCUUAagGGCCA--UUCGGCGGCUgg -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 36247 | 0.67 | 0.788333 |
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Target: 5'- gCGGAGUgguuuUCCCGGUcAGUugcguaucgaCGCCGuacGCCg -3' miRNA: 3'- gGUCUUA-----AGGGCCAuUCG----------GCGGC---UGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 54686 | 0.66 | 0.800928 |
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Target: 5'- aCCGGGAuUUCCCGcagcgauuuuccguGCCGUCGGCUu -3' miRNA: 3'- -GGUCUU-AAGGGCcauu----------CGGCGGCUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 35799 | 0.66 | 0.816022 |
|
Target: 5'- gCCAGcAUUgagccuuucgucgCCCGcGUAGaucguugcGUCGCCGGCCa -3' miRNA: 3'- -GGUCuUAA-------------GGGC-CAUU--------CGGCGGCUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 49815 | 0.66 | 0.834194 |
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Target: 5'- uUCAGAAUuuggccuUCCuCGGUGAGuuGaagcCCGuACCg -3' miRNA: 3'- -GGUCUUA-------AGG-GCCAUUCggC----GGC-UGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 51132 | 0.66 | 0.835081 |
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Target: 5'- gCCAcAAUUUCCuuauAGCUGUCGACCa -3' miRNA: 3'- -GGUcUUAAGGGccauUCGGCGGCUGG- -5' |
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| 24824 | 3' | -53.8 | NC_005284.1 | + | 44583 | 1.15 | 0.000689 |
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Target: 5'- gCCAGAAUUCCCGGUAAGCCGCCGACCg -3' miRNA: 3'- -GGUCUUAAGGGCCAUUCGGCGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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