Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24932 | 3' | -57.8 | NC_005284.1 | + | 29467 | 0.67 | 0.51506 |
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Target: 5'- gACGAcGAAACaAGGcuugucgaGCGACUGGCGgaagaGCGg -3' miRNA: 3'- -UGCU-CUUUG-UCC--------CGCUGGCCGCa----CGC- -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 22133 | 0.66 | 0.556808 |
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Target: 5'- cACGG---ACAGuggaaaGGCGACCGGCaUGCGu -3' miRNA: 3'- -UGCUcuuUGUC------CCGCUGGCCGcACGC- -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 19015 | 0.66 | 0.556808 |
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Target: 5'- gGCGGGGugauUAGGuCGAUCGGCGcGCGc -3' miRNA: 3'- -UGCUCUuu--GUCCcGCUGGCCGCaCGC- -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 7037 | 0.66 | 0.556808 |
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Target: 5'- cACGAcGAAAUAcGGCG-CCGGCGccuucuccggUGCGa -3' miRNA: 3'- -UGCU-CUUUGUcCCGCuGGCCGC----------ACGC- -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 12906 | 0.66 | 0.599473 |
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Target: 5'- gACGaAGAGuccGCGGGcGCGAagCGGCG-GCGu -3' miRNA: 3'- -UGC-UCUU---UGUCC-CGCUg-GCCGCaCGC- -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 34199 | 0.67 | 0.49466 |
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Target: 5'- uACGAc---CAuGuGGCGAUCGGCGUGCa -3' miRNA: 3'- -UGCUcuuuGU-C-CCGCUGGCCGCACGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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