Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24950 | 5' | -52.6 | NC_005284.1 | + | 16013 | 0.67 | 0.761649 |
Target: 5'- -aGCUCGagcAUCCggUCGGGCGcGACGa -3' miRNA: 3'- aaCGAGCgu-UGGGa-AGCUCGCuUUGC- -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 28657 | 0.67 | 0.782414 |
Target: 5'- gUGUUCGCAGcguacaucuucCCCUUCcGGGUGuGGACGa -3' miRNA: 3'- aACGAGCGUU-----------GGGAAG-CUCGC-UUUGC- -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 12536 | 0.67 | 0.789531 |
Target: 5'- -gGCUCGCGggguucgacgauccGCCC-UCGAuCGAAACu -3' miRNA: 3'- aaCGAGCGU--------------UGGGaAGCUcGCUUUGc -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 29941 | 0.66 | 0.802518 |
Target: 5'- gUGCUgCGUuuGCCCgugucuugaCGGGCGAGACa -3' miRNA: 3'- aACGA-GCGu-UGGGaa-------GCUCGCUUUGc -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 37637 | 0.66 | 0.802518 |
Target: 5'- cUGCUCGCcguGCCgUUCGuGUGGcuGGCa -3' miRNA: 3'- aACGAGCGu--UGGgAAGCuCGCU--UUGc -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 25666 | 0.66 | 0.821865 |
Target: 5'- -gGCggCGCAGCCgUUCGcGCGAGcCa -3' miRNA: 3'- aaCGa-GCGUUGGgAAGCuCGCUUuGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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