Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24951 | 3' | -62.7 | NC_005284.1 | + | 40176 | 0.66 | 0.314134 |
Target: 5'- gAACCGCCACgugCGUCGcgaccaacuccGGCGUCCucugGCUCc -3' miRNA: 3'- -UUGGCGGUG---GCGGC-----------UCGCAGG----CGAGa -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 37077 | 0.66 | 0.314134 |
Target: 5'- cGACCGCCAUcgacgCGCCGgcgagauucagGGCGUCgCGCa-- -3' miRNA: 3'- -UUGGCGGUG-----GCGGC-----------UCGCAG-GCGaga -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 14582 | 0.66 | 0.32163 |
Target: 5'- uGGCUGCUGCgCGUCGGaUGUCUGCUCg -3' miRNA: 3'- -UUGGCGGUG-GCGGCUcGCAGGCGAGa -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 48260 | 0.66 | 0.337022 |
Target: 5'- gAGCUGUCACCGCaaCGGGCGUCacuuCGC-Ca -3' miRNA: 3'- -UUGGCGGUGGCG--GCUCGCAG----GCGaGa -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 15916 | 0.66 | 0.344917 |
Target: 5'- -uCCGaaaCGCaCGCCGAGCGUgCCGUa-- -3' miRNA: 3'- uuGGCg--GUG-GCGGCUCGCA-GGCGaga -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 13682 | 0.66 | 0.352943 |
Target: 5'- cGACCGUCGCC-CCGAGCuGgccagCCGUcuUCUu -3' miRNA: 3'- -UUGGCGGUGGcGGCUCG-Ca----GGCG--AGA- -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 9565 | 0.66 | 0.352943 |
Target: 5'- cGCUGUCGCCGCCu-GCGUcgCCGCg-- -3' miRNA: 3'- uUGGCGGUGGCGGcuCGCA--GGCGaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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