Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25023 | 3' | -46.6 | NC_005309.1 | + | 201101 | 1.01 | 0.146777 |
Target: 5'- cAGUACCUACUUCUAAAAUCACCCCc- -3' miRNA: 3'- -UCAUGGAUGAAGAUUUUAGUGGGGcg -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 258200 | 1.04 | 0.101531 |
Target: 5'- uAGUACCUACUUCUAAAAUCACCCgGCc -3' miRNA: 3'- -UCAUGGAUGAAGAUUUUAGUGGGgCG- -5' |
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25023 | 3' | -46.6 | NC_005309.1 | + | 260372 | 1.15 | 0.026618 |
Target: 5'- uAGUACCUACUUCUAAAAUCACCCCGCg -3' miRNA: 3'- -UCAUGGAUGAAGAUUUUAGUGGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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