miRNA: 3'- ucAUGGAUGAAGAU-------UUUAGUGGg----GCG- -5'
miRNA: 3'- uCAUGGAUgaAGAUuuUAGUGGGGCG- -5'
miRNA: 3'- -UCAUGG--AUGAAGAUuuuaGugGGGCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25023 | 3' | -46.6 | NC_005309.1 | + | 347899 | 0.7 | 0.999855 |
|
Target: 5'- -aUACCUACUUCUAucguaugGAAUCGCUauugaCGUa -3' miRNA: 3'- ucAUGGAUGAAGAU-------UUUAGUGGg----GCG- -5' |
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| 25023 | 3' | -46.6 | NC_005309.1 | + | 354162 | 0.72 | 0.999311 |
|
Target: 5'- uGUAUCUGgaUCUG--GUCACCCUGUu -3' miRNA: 3'- uCAUGGAUgaAGAUuuUAGUGGGGCG- -5' |
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| 25023 | 3' | -46.6 | NC_005309.1 | + | 354342 | 0.69 | 0.999951 |
|
Target: 5'- uGGUGCCguUGCUUCUG----UugCCCGUg -3' miRNA: 3'- -UCAUGG--AUGAAGAUuuuaGugGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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