Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25027 | 3' | -48.6 | NC_005336.1 | + | 93883 | 0.66 | 0.997219 |
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Target: 5'- -aGUGCGcuuucuGGACgaagaGGAGGAGCu -3' miRNA: 3'- aaCACGCu-----UUUGaaaagCCUCCUCGc -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 110616 | 0.66 | 0.997219 |
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Target: 5'- ---cGCGAcGACgagcUCGGAGGGcGCGa -3' miRNA: 3'- aacaCGCUuUUGaaa-AGCCUCCU-CGC- -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 117458 | 0.66 | 0.997672 |
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Target: 5'- cUGUGCGGAAGCgugcacUCGGcgcaauggaucGGcGAGCGc -3' miRNA: 3'- aACACGCUUUUGaaa---AGCC-----------UC-CUCGC- -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 18261 | 0.66 | 0.997951 |
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Target: 5'- ---cGCGAGGGCUUggagUUgggcauggcgagcgGGGGGAGCGc -3' miRNA: 3'- aacaCGCUUUUGAAa---AG--------------CCUCCUCGC- -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 18586 | 0.66 | 0.998062 |
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Target: 5'- ---aGCGGu-GCcugUCGGAGGAGUGu -3' miRNA: 3'- aacaCGCUuuUGaaaAGCCUCCUCGC- -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 36099 | 0.66 | 0.998062 |
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Target: 5'- -cGUGCGGAAGCgcugcaCGGAGucGCa -3' miRNA: 3'- aaCACGCUUUUGaaaa--GCCUCcuCGc -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 99934 | 0.66 | 0.998395 |
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Target: 5'- cUGaUGCGGcgGCUgcgugCGGAGG-GCGc -3' miRNA: 3'- aAC-ACGCUuuUGAaaa--GCCUCCuCGC- -5' |
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| 25027 | 3' | -48.6 | NC_005336.1 | + | 32407 | 0.66 | 0.998395 |
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Target: 5'- -cGUGCGGcaGAGCgugacggcUUGGGGGAuGCGg -3' miRNA: 3'- aaCACGCU--UUUGaaa-----AGCCUCCU-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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