Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25030 | 3' | -54.3 | NC_005336.1 | + | 10193 | 0.66 | 0.940494 |
Target: 5'- uGCaUCACUCGCUCGgUgACguuCUCGu -3' miRNA: 3'- uUG-AGUGAGCGAGCgAgUGaguGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 9427 | 0.66 | 0.940494 |
Target: 5'- gAGCgCGCUCggGCUCGC-CGagaacCUCGCUCGg -3' miRNA: 3'- -UUGaGUGAG--CGAGCGaGU-----GAGUGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 124039 | 0.66 | 0.940494 |
Target: 5'- cGACugUCGCgUCGCgcggcCGCggCGCUCGCUCa -3' miRNA: 3'- -UUG--AGUG-AGCGa----GCGa-GUGAGUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 137045 | 0.66 | 0.935579 |
Target: 5'- uAACUCACUCaCUCuCUCACUCGg--- -3' miRNA: 3'- -UUGAGUGAGcGAGcGAGUGAGUgagc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 137045 | 0.66 | 0.935579 |
Target: 5'- uAACUCACUCaCUCuCUCACUCGg--- -3' miRNA: 3'- -UUGAGUGAGcGAGcGAGUGAGUgagc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 122553 | 0.66 | 0.930414 |
Target: 5'- cGCgcgCGCUCGCcagcCGCgCGCUCgACUCGu -3' miRNA: 3'- uUGa--GUGAGCGa---GCGaGUGAG-UGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 47301 | 0.67 | 0.913412 |
Target: 5'- -cCUC-CUCGCucUCGCUCucaUCGCUCu -3' miRNA: 3'- uuGAGuGAGCG--AGCGAGug-AGUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 43479 | 0.67 | 0.912806 |
Target: 5'- -cCUCACUgGCcgcgguaUCGUgcgCACUCGCUCc -3' miRNA: 3'- uuGAGUGAgCG-------AGCGa--GUGAGUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 117723 | 0.67 | 0.907244 |
Target: 5'- aGACUCACgaaguaGCUcCGC-CACUC-CUCGu -3' miRNA: 3'- -UUGAGUGag----CGA-GCGaGUGAGuGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 106341 | 0.67 | 0.885861 |
Target: 5'- gGGCUCAUcucgCGCUCGCggcugCACgacuaccucaugCGCUCGa -3' miRNA: 3'- -UUGAGUGa---GCGAGCGa----GUGa-----------GUGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 136850 | 0.67 | 0.884443 |
Target: 5'- cACUCACUCggaguaagcgaguGCgaacaggucaacagUCGaCUCACUCACUCu -3' miRNA: 3'- uUGAGUGAG-------------CG--------------AGC-GAGUGAGUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 136850 | 0.67 | 0.884443 |
Target: 5'- cACUCACUCggaguaagcgaguGCgaacaggucaacagUCGaCUCACUCACUCu -3' miRNA: 3'- uUGAGUGAG-------------CG--------------AGC-GAGUGAGUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 130441 | 0.67 | 0.880133 |
Target: 5'- cGCUCACgCGCgggcUGCUCGCgaucgCGCUCu -3' miRNA: 3'- uUGAGUGaGCGa---GCGAGUGa----GUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 11726 | 0.68 | 0.872764 |
Target: 5'- cGCgugCGCUCGCgCGCUCuccgCACUCa -3' miRNA: 3'- uUGa--GUGAGCGaGCGAGuga-GUGAGc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 59240 | 0.69 | 0.832647 |
Target: 5'- -uCUCGCcCGCgCGCUUcgcggcguGCUCGCUCGa -3' miRNA: 3'- uuGAGUGaGCGaGCGAG--------UGAGUGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 137651 | 0.69 | 0.824019 |
Target: 5'- uAAC-CACUCGCgCaCUC-CUCACUCGg -3' miRNA: 3'- -UUGaGUGAGCGaGcGAGuGAGUGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 137651 | 0.69 | 0.824019 |
Target: 5'- uAAC-CACUCGCgCaCUC-CUCACUCGg -3' miRNA: 3'- -UUGaGUGAGCGaGcGAGuGAGUGAGC- -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 138114 | 0.71 | 0.69852 |
Target: 5'- cACUCACUCGCUCGCUCGg------- -3' miRNA: 3'- uUGAGUGAGCGAGCGAGUgagugagc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 138114 | 0.71 | 0.69852 |
Target: 5'- cACUCACUCGCUCGCUCGg------- -3' miRNA: 3'- uUGAGUGAGCGAGCGAGUgagugagc -5' |
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25030 | 3' | -54.3 | NC_005336.1 | + | 46849 | 0.72 | 0.646426 |
Target: 5'- uGAC-CACgCGCUUGUUCAgCUCGCUCGc -3' miRNA: 3'- -UUGaGUGaGCGAGCGAGU-GAGUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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