Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25031 | 3' | -45.5 | NC_005336.1 | + | 1841 | 0.66 | 0.999969 |
Target: 5'- -cGACuGUcGACUCGuuaacugucCUCuACUCUCUCa -3' miRNA: 3'- uaUUGuCAaUUGAGU---------GAG-UGAGAGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 1841 | 0.66 | 0.999969 |
Target: 5'- -cGACuGUcGACUCGuuaacugucCUCuACUCUCUCa -3' miRNA: 3'- uaUUGuCAaUUGAGU---------GAG-UGAGAGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 137770 | 0.66 | 0.999942 |
Target: 5'- uUAACAGguuaaUCACUCACUCa--- -3' miRNA: 3'- uAUUGUCaauugAGUGAGUGAGagag -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 137770 | 0.66 | 0.999942 |
Target: 5'- uUAACAGguuaaUCACUCACUCa--- -3' miRNA: 3'- uAUUGUCaauugAGUGAGUGAGagag -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 44986 | 0.66 | 0.999942 |
Target: 5'- cUGGCAGUUcccGGCgCcCUCGCUCUCa- -3' miRNA: 3'- uAUUGUCAA---UUGaGuGAGUGAGAGag -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 128703 | 0.67 | 0.999821 |
Target: 5'- -gAACAGUaugcuACUCACacagUCGCUC-CUCg -3' miRNA: 3'- uaUUGUCAau---UGAGUG----AGUGAGaGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 136802 | 0.67 | 0.999821 |
Target: 5'- --cGCGG---ACUCACUCACUCUg-- -3' miRNA: 3'- uauUGUCaauUGAGUGAGUGAGAgag -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 136802 | 0.67 | 0.999821 |
Target: 5'- --cGCGG---ACUCACUCACUCUg-- -3' miRNA: 3'- uauUGUCaauUGAGUGAGUGAGAgag -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 103214 | 0.68 | 0.999388 |
Target: 5'- --cGCAGgucgGGCUCAuCUCGCagCUCUCc -3' miRNA: 3'- uauUGUCaa--UUGAGU-GAGUGa-GAGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 137642 | 0.69 | 0.998828 |
Target: 5'- uUAGCgAGUUAaccACUCGCgCACUC-CUCa -3' miRNA: 3'- uAUUG-UCAAU---UGAGUGaGUGAGaGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 137642 | 0.69 | 0.998828 |
Target: 5'- uUAGCgAGUUAaccACUCGCgCACUC-CUCa -3' miRNA: 3'- uAUUG-UCAAU---UGAGUGaGUGAGaGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 77618 | 0.69 | 0.998828 |
Target: 5'- -aAGCGGUccACaaACUCGCUCUCUa -3' miRNA: 3'- uaUUGUCAauUGagUGAGUGAGAGAg -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 1737 | 0.69 | 0.998248 |
Target: 5'- uUAACuuaUUAACUCGucCUCuACUCUCUCa -3' miRNA: 3'- uAUUGuc-AAUUGAGU--GAG-UGAGAGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 1737 | 0.69 | 0.998248 |
Target: 5'- uUAACuuaUUAACUCGucCUCuACUCUCUCa -3' miRNA: 3'- uAUUGuc-AAUUGAGU--GAG-UGAGAGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 136835 | 0.7 | 0.997444 |
Target: 5'- --uACcGUUGACUCACgCACUCaCUCg -3' miRNA: 3'- uauUGuCAAUUGAGUGaGUGAGaGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 136835 | 0.7 | 0.997444 |
Target: 5'- --uACcGUUGACUCACgCACUCaCUCg -3' miRNA: 3'- uauUGuCAAUUGAGUGaGUGAGaGAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 137842 | 0.7 | 0.994908 |
Target: 5'- uUAACAGguuaaUCACUCACUCacaCUCa -3' miRNA: 3'- uAUUGUCaauugAGUGAGUGAGa--GAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 137842 | 0.7 | 0.994908 |
Target: 5'- uUAACAGguuaaUCACUCACUCacaCUCa -3' miRNA: 3'- uAUUGUCaauugAGUGAGUGAGa--GAG- -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 136979 | 0.76 | 0.926269 |
Target: 5'- -cAACAGgcAACUCGCUCGCUCg--- -3' miRNA: 3'- uaUUGUCaaUUGAGUGAGUGAGagag -5' |
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25031 | 3' | -45.5 | NC_005336.1 | + | 136979 | 0.76 | 0.926269 |
Target: 5'- -cAACAGgcAACUCGCUCGCUCg--- -3' miRNA: 3'- uaUUGUCaaUUGAGUGAGUGAGagag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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