Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25031 | 5' | -47.1 | NC_005336.1 | + | 9619 | 0.66 | 0.999524 |
Target: 5'- gGAGGGGAAGAugcGCGCGAGcgCGGcCUGg- -3' miRNA: 3'- -CUCUCUUUCU---CGUGUUCa-GUU-GACaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 34463 | 0.66 | 0.999251 |
Target: 5'- -cGAGAccGAGCACG-GUCAGCg--- -3' miRNA: 3'- cuCUCUuuCUCGUGUuCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 2477 | 0.67 | 0.998853 |
Target: 5'- uGAGcGAGuGAGUgaGCGAGUUGACUGUUc -3' miRNA: 3'- -CUCuCUUuCUCG--UGUUCAGUUGACAA- -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 136955 | 0.67 | 0.998853 |
Target: 5'- aAGAGuAAGAGCGCGauaacaAGUCAACa--- -3' miRNA: 3'- cUCUCuUUCUCGUGU------UCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 136955 | 0.67 | 0.998853 |
Target: 5'- aAGAGuAAGAGCGCGauaacaAGUCAACa--- -3' miRNA: 3'- cUCUCuUUCUCGUGU------UCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 2477 | 0.67 | 0.998853 |
Target: 5'- uGAGcGAGuGAGUgaGCGAGUUGACUGUUc -3' miRNA: 3'- -CUCuCUUuCUCG--UGUUCAGUUGACAA- -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 137998 | 0.67 | 0.998255 |
Target: 5'- gGAGuGAGAGAGUagagaacacgauaACGAGUCGACa--- -3' miRNA: 3'- -CUCuCUUUCUCG-------------UGUUCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 137998 | 0.67 | 0.998255 |
Target: 5'- gGAGuGAGAGAGUagagaacacgauaACGAGUCGACa--- -3' miRNA: 3'- -CUCuCUUUCUCG-------------UGUUCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 138057 | 0.67 | 0.997928 |
Target: 5'- uGAGAGAGuAGAGgACGAGUUAAUa--- -3' miRNA: 3'- -CUCUCUU-UCUCgUGUUCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 126146 | 0.67 | 0.997928 |
Target: 5'- aGGAGGAAGAGCGCGAcGUCcucGGCg--- -3' miRNA: 3'- cUCUCUUUCUCGUGUU-CAG---UUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 138057 | 0.67 | 0.997928 |
Target: 5'- uGAGAGAGuAGAGgACGAGUUAAUa--- -3' miRNA: 3'- -CUCUCUU-UCUCgUGUUCAGUUGacaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 9498 | 0.68 | 0.997013 |
Target: 5'- -cGAGGAAGAGCGCAGccUCGACcaUGUUc -3' miRNA: 3'- cuCUCUUUCUCGUGUUc-AGUUG--ACAA- -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 137944 | 0.68 | 0.996444 |
Target: 5'- gGAGuGAGAGAGUagaggacaguuaACGAGUCGACaGUc -3' miRNA: 3'- -CUCuCUUUCUCG------------UGUUCAGUUGaCAa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 137944 | 0.68 | 0.996444 |
Target: 5'- gGAGuGAGAGAGUagaggacaguuaACGAGUCGACaGUc -3' miRNA: 3'- -CUCuCUUUCUCG------------UGUUCAGUUGaCAa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 139107 | 0.69 | 0.993205 |
Target: 5'- cGGAGGAgcgcGGAGgGCGGGUCAGCUc-- -3' miRNA: 3'- cUCUCUU----UCUCgUGUUCAGUUGAcaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 139107 | 0.69 | 0.993205 |
Target: 5'- cGGAGGAgcgcGGAGgGCGGGUCAGCUc-- -3' miRNA: 3'- cUCUCUU----UCUCgUGUUCAGUUGAcaa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 137232 | 0.69 | 0.990871 |
Target: 5'- cGAGAGAAcGGAuaGCGAGUCGACaGUc -3' miRNA: 3'- -CUCUCUU-UCUcgUGUUCAGUUGaCAa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 137232 | 0.69 | 0.990871 |
Target: 5'- cGAGAGAAcGGAuaGCGAGUCGACaGUc -3' miRNA: 3'- -CUCUCUU-UCUcgUGUUCAGUUGaCAa -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 2919 | 0.71 | 0.977489 |
Target: 5'- cGAGAGu--GAGU--GAGUCGACUGUUg -3' miRNA: 3'- -CUCUCuuuCUCGugUUCAGUUGACAA- -5' |
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25031 | 5' | -47.1 | NC_005336.1 | + | 2919 | 0.71 | 0.977489 |
Target: 5'- cGAGAGu--GAGU--GAGUCGACUGUUg -3' miRNA: 3'- -CUCUCuuuCUCGugUUCAGUUGACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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