Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25034 | 5' | -51.1 | NC_005336.1 | + | 88676 | 0.66 | 0.991961 |
Target: 5'- gUACCGagugACCAGCgCGagcGAGGAgccgacCUCCCu -3' miRNA: 3'- gAUGGUa---UGGUUG-GU---CUCCUa-----GAGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 14066 | 0.66 | 0.99078 |
Target: 5'- -gGCCGcGCCGGCCucGAGcaccuGGUCUCCg -3' miRNA: 3'- gaUGGUaUGGUUGGu-CUC-----CUAGAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 81197 | 0.66 | 0.99078 |
Target: 5'- -cGCCGUGCU-GCgGGGGGA-CUCCg -3' miRNA: 3'- gaUGGUAUGGuUGgUCUCCUaGAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 129485 | 0.66 | 0.989466 |
Target: 5'- -cGCCaAUAUCGACgUGGAGGuUCUCCg -3' miRNA: 3'- gaUGG-UAUGGUUG-GUCUCCuAGAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 106448 | 0.66 | 0.989466 |
Target: 5'- --cCCGUACC-ACCAGuacauGGGAaCUCCg -3' miRNA: 3'- gauGGUAUGGuUGGUC-----UCCUaGAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 78620 | 0.66 | 0.989466 |
Target: 5'- gUGCgCAcGCCGggcgucuuGCC-GAGGAUCUCgCCg -3' miRNA: 3'- gAUG-GUaUGGU--------UGGuCUCCUAGAG-GG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 85009 | 0.66 | 0.988901 |
Target: 5'- gCUACCAccugacguacaugACCGACCucuGGAagUCCCa -3' miRNA: 3'- -GAUGGUa------------UGGUUGGucuCCUagAGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 10725 | 0.66 | 0.98861 |
Target: 5'- -gGCC-UGCCGGCCAG-GGAcgccgccacacaccuUCcCCCa -3' miRNA: 3'- gaUGGuAUGGUUGGUCuCCU---------------AGaGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 105533 | 0.66 | 0.986404 |
Target: 5'- -cGCCGcGCgCGugcGCCGGGGGcaggCUCCCg -3' miRNA: 3'- gaUGGUaUG-GU---UGGUCUCCua--GAGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 75130 | 0.67 | 0.984637 |
Target: 5'- -cACCAgcgcggACCAGCCGGAGucgucCUCCa -3' miRNA: 3'- gaUGGUa-----UGGUUGGUCUCcua--GAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 118746 | 0.67 | 0.984637 |
Target: 5'- -cAgCAUGCCaAGCguGAGGcuaCUCCCg -3' miRNA: 3'- gaUgGUAUGG-UUGguCUCCua-GAGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 89622 | 0.67 | 0.984263 |
Target: 5'- -cGCCAU-CCGACCGGAGGuugagguugcgCUUCa -3' miRNA: 3'- gaUGGUAuGGUUGGUCUCCua---------GAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 14335 | 0.67 | 0.9827 |
Target: 5'- --cCCAUcGCagcgaGGCCAGGcGGAUCUCCg -3' miRNA: 3'- gauGGUA-UGg----UUGGUCU-CCUAGAGGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 26950 | 0.67 | 0.9827 |
Target: 5'- -cGCCGgguCCAGgCAGAccgGGAUCUCgCg -3' miRNA: 3'- gaUGGUau-GGUUgGUCU---CCUAGAGgG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 131110 | 0.67 | 0.980586 |
Target: 5'- -gGCCAUGCUGACCuGGGGcgUgcgCCa -3' miRNA: 3'- gaUGGUAUGGUUGGuCUCCuaGa--GGg -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 9091 | 0.67 | 0.980586 |
Target: 5'- gCUACUcgGCCAGuCCGGAGG----CCCg -3' miRNA: 3'- -GAUGGuaUGGUU-GGUCUCCuagaGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 74063 | 0.67 | 0.980586 |
Target: 5'- -gGCCGUcgagguCUAGCgCGG-GGAUCUCCUg -3' miRNA: 3'- gaUGGUAu-----GGUUG-GUCuCCUAGAGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 21872 | 0.67 | 0.975786 |
Target: 5'- aCU-CCAUGCUggUguGAGGGUagucgaUCCCc -3' miRNA: 3'- -GAuGGUAUGGuuGguCUCCUAg-----AGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 79697 | 0.68 | 0.973084 |
Target: 5'- ---aCAgcuCCucGCCGGAGG-UCUCCCa -3' miRNA: 3'- gaugGUau-GGu-UGGUCUCCuAGAGGG- -5' |
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25034 | 5' | -51.1 | NC_005336.1 | + | 107321 | 0.68 | 0.973084 |
Target: 5'- uUACCAguuuccCCAACUuugGGAGGAaCUUCCu -3' miRNA: 3'- gAUGGUau----GGUUGG---UCUCCUaGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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