Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25037 | 3' | -54 | NC_005336.1 | + | 47671 | 0.66 | 0.964549 |
Target: 5'- gAGUGcg-CCGCCGgcagCGUCGACgCGGa -3' miRNA: 3'- -UCACaaaGGCGGCag--GUAGCUG-GUCc -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 138767 | 0.66 | 0.957472 |
Target: 5'- --cGgg-CCGCCG-CCcgCGACCGcGGg -3' miRNA: 3'- ucaCaaaGGCGGCaGGuaGCUGGU-CC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 138767 | 0.66 | 0.957472 |
Target: 5'- --cGgg-CCGCCG-CCcgCGACCGcGGg -3' miRNA: 3'- ucaCaaaGGCGGCaGGuaGCUGGU-CC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 29918 | 0.66 | 0.953595 |
Target: 5'- gGGaUGU---UGCUG-CCGUCGGCCAGGc -3' miRNA: 3'- -UC-ACAaagGCGGCaGGUAGCUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 51196 | 0.66 | 0.949486 |
Target: 5'- --gGUcUCCGCCGUCUA-CGGCUucaccgAGGa -3' miRNA: 3'- ucaCAaAGGCGGCAGGUaGCUGG------UCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 98436 | 0.66 | 0.949486 |
Target: 5'- --cGgggUCGCCGUCC-UCGACCAc- -3' miRNA: 3'- ucaCaaaGGCGGCAGGuAGCUGGUcc -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 17832 | 0.67 | 0.945142 |
Target: 5'- uGGcUGgggaUGCCGUCgGccUCGGCCAGGg -3' miRNA: 3'- -UC-ACaaagGCGGCAGgU--AGCUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 50626 | 0.67 | 0.945142 |
Target: 5'- -----aUCUGCuuuCGUUgGUCGACCAGGa -3' miRNA: 3'- ucacaaAGGCG---GCAGgUAGCUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 4333 | 0.67 | 0.940561 |
Target: 5'- -uUGUUUCCGUUugcagCCGUCaGCCAGGc -3' miRNA: 3'- ucACAAAGGCGGca---GGUAGcUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 26943 | 0.67 | 0.930676 |
Target: 5'- aGGUGg--CCGCagaGaucUCCGUCG-CCGGGa -3' miRNA: 3'- -UCACaaaGGCGg--C---AGGUAGCuGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 47046 | 0.67 | 0.930676 |
Target: 5'- uGGUGcggcgCgCGCCGUCCccGUgGAUCGGGa -3' miRNA: 3'- -UCACaaa--G-GCGGCAGG--UAgCUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 50273 | 0.67 | 0.930676 |
Target: 5'- cGGgcag-CCGCCG-CCGUCGGCCGc- -3' miRNA: 3'- -UCacaaaGGCGGCaGGUAGCUGGUcc -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 3232 | 0.67 | 0.919822 |
Target: 5'- gAGuUGUagCCGCCGaCCAccgCGACgCAGGu -3' miRNA: 3'- -UC-ACAaaGGCGGCaGGUa--GCUG-GUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 3232 | 0.67 | 0.919822 |
Target: 5'- gAGuUGUagCCGCCGaCCAccgCGACgCAGGu -3' miRNA: 3'- -UC-ACAaaGGCGGCaGGUa--GCUG-GUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 40482 | 0.68 | 0.895225 |
Target: 5'- aGGUGUggaagagCCGCgCGUCCG-CGugCAuGGa -3' miRNA: 3'- -UCACAaa-----GGCG-GCAGGUaGCugGU-CC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 98486 | 0.68 | 0.888486 |
Target: 5'- -uUGUUUCCGgCGgcgaCAUCGACCAc- -3' miRNA: 3'- ucACAAAGGCgGCag--GUAGCUGGUcc -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 103707 | 0.69 | 0.874325 |
Target: 5'- cGUGauggCCGUgUGUCCcuUCGACCAGGa -3' miRNA: 3'- uCACaaa-GGCG-GCAGGu-AGCUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 41469 | 0.69 | 0.859286 |
Target: 5'- ------aCCGCCG-CCGUCGAagaaCCAGGa -3' miRNA: 3'- ucacaaaGGCGGCaGGUAGCU----GGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 26947 | 0.7 | 0.835183 |
Target: 5'- -----gUCCGCCGggUCCAggcaGACCGGGa -3' miRNA: 3'- ucacaaAGGCGGC--AGGUag--CUGGUCC- -5' |
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25037 | 3' | -54 | NC_005336.1 | + | 124975 | 0.7 | 0.818166 |
Target: 5'- cGUGc-UCUGCCG-CCAgauggCGGCCGGGu -3' miRNA: 3'- uCACaaAGGCGGCaGGUa----GCUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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