Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25037 | 5' | -56.5 | NC_005336.1 | + | 64699 | 0.66 | 0.870692 |
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Target: 5'- uUCCUGGacuucgaguUCGCC-----GGCGGAGACg -3' miRNA: 3'- cAGGAUC---------AGCGGuugcaCCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 62054 | 0.66 | 0.870692 |
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Target: 5'- cGUCaUGGUCGCCAGCGagccgcGGCGcGcGGCc -3' miRNA: 3'- -CAGgAUCAGCGGUUGCa-----CCGC-CuCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 76412 | 0.66 | 0.870692 |
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Target: 5'- gGUCCUugaAGUCuGCgAGgaUGGCGGAGAa -3' miRNA: 3'- -CAGGA---UCAG-CGgUUgcACCGCCUCUg -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 40194 | 0.66 | 0.877979 |
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Target: 5'- -aCCcGGUCGCC-GCGc-GCGGAGAUc -3' miRNA: 3'- caGGaUCAGCGGuUGCacCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 65977 | 0.66 | 0.877979 |
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Target: 5'- -aCCgcGUCGCgGcCGUGGCcggcgccguGGAGGCg -3' miRNA: 3'- caGGauCAGCGgUuGCACCG---------CCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 88597 | 0.66 | 0.885044 |
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Target: 5'- uGUCCUcGGUgaaGCCGuagacGGCGGAGACc -3' miRNA: 3'- -CAGGA-UCAg--CGGUugca-CCGCCUCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 75300 | 0.66 | 0.885044 |
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Target: 5'- uGUCCacaaAGUCGUCgAGCGUGuCGGuGGCg -3' miRNA: 3'- -CAGGa---UCAGCGG-UUGCACcGCCuCUG- -5' |
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| 25037 | 5' | -56.5 | NC_005336.1 | + | 128105 | 0.66 | 0.885044 |
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Target: 5'- aUCCg---CGCCuGCGaGGCGGAGGu -3' miRNA: 3'- cAGGaucaGCGGuUGCaCCGCCUCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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