Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
252 | 5' | -59.1 | AC_000008.1 | + | 10459 | 0.66 | 0.345459 |
Target: 5'- cGGC-GGCGGuGGUggaGGCGC-GCGGAAa -3' miRNA: 3'- -UCGuCCGUCuCCG---CCGCGaCGCUUUc -5' |
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252 | 5' | -59.1 | AC_000008.1 | + | 8530 | 0.66 | 0.354009 |
Target: 5'- cGCGcGGCGGGcGGUGG-GCcGCGggGGu -3' miRNA: 3'- uCGU-CCGUCU-CCGCCgCGaCGCuuUC- -5' |
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252 | 5' | -59.1 | AC_000008.1 | + | 20666 | 0.66 | 0.362707 |
Target: 5'- cGCA-GCGGGGGCGGCaGCUuCGGc-- -3' miRNA: 3'- uCGUcCGUCUCCGCCG-CGAcGCUuuc -5' |
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252 | 5' | -59.1 | AC_000008.1 | + | 2175 | 0.66 | 0.371553 |
Target: 5'- aAGcCAGGCGGcGGCGGCaGgaGC-AGAGc -3' miRNA: 3'- -UC-GUCCGUCuCCGCCG-CgaCGcUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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