Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2533 | 3' | -50 | NC_001460.1 | + | 2007 | 0.66 | 0.879107 |
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Target: 5'- aGCGGCGGcaUGGUC-AAagGcCGC-GCCa -3' miRNA: 3'- -CGCUGUC--ACCAGaUUuaCaGCGuCGG- -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 15407 | 0.66 | 0.870783 |
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Target: 5'- cGCGgaGCAGcuaGGUUUGcAAuagcUGUUGCGGCCg -3' miRNA: 3'- -CGC--UGUCa--CCAGAU-UU----ACAGCGUCGG- -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 3154 | 0.66 | 0.862175 |
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Target: 5'- cCGACAGUG---UGAGUGUgGUAGCa -3' miRNA: 3'- cGCUGUCACcagAUUUACAgCGUCGg -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 17742 | 0.67 | 0.825096 |
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Target: 5'- uGCcGCAGUGGUCUuacAUGcacaUCGcCGGUCa -3' miRNA: 3'- -CGcUGUCACCAGAuu-UAC----AGC-GUCGG- -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 30599 | 0.68 | 0.794809 |
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Target: 5'- aGUGuCAGagGGUCguaugGAGUGUUGCuGCUg -3' miRNA: 3'- -CGCuGUCa-CCAGa----UUUACAGCGuCGG- -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 15254 | 0.69 | 0.729358 |
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Target: 5'- aUGGCGGUGGUUg----GUCuacgccggcgacGCAGCCg -3' miRNA: 3'- cGCUGUCACCAGauuuaCAG------------CGUCGG- -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 17476 | 0.71 | 0.612809 |
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Target: 5'- cGCGAUAGUGGguUCUGGcaaagGagGCAGCUc -3' miRNA: 3'- -CGCUGUCACC--AGAUUua---CagCGUCGG- -5' |
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| 2533 | 3' | -50 | NC_001460.1 | + | 26374 | 1.16 | 0.000682 |
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Target: 5'- gGCGACAGUGGUCUAAAUGUCGCAGCCg -3' miRNA: 3'- -CGCUGUCACCAGAUUUACAGCGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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