Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25508 | 5' | -55.6 | NC_005337.1 | + | 116281 | 0.68 | 0.808707 |
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Target: 5'- uCCGCCgGUGAUCgAGGaguaccuCGGCUCccgccccuccAUGGAGc -3' miRNA: 3'- -GGCGG-CACUAG-UCC-------GCCGAG----------UAUCUC- -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 117643 | 0.66 | 0.914031 |
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Target: 5'- aCCGCCGUGGUgGaGC-GCUCGaAGAa -3' miRNA: 3'- -GGCGGCACUAgUcCGcCGAGUaUCUc -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 119786 | 0.73 | 0.541263 |
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Target: 5'- gCCGCgCGUGGUCGGuGCGGUgguggcgagggUgGUGGAGa -3' miRNA: 3'- -GGCG-GCACUAGUC-CGCCG-----------AgUAUCUC- -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 123605 | 0.67 | 0.881573 |
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Target: 5'- gCCGCaCGUGccgcgcucgCGGGUGcugacGCUCGUGGAGc -3' miRNA: 3'- -GGCG-GCACua-------GUCCGC-----CGAGUAUCUC- -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 124316 | 0.71 | 0.643292 |
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Target: 5'- uCCGUCGcgGcgCAGGCG-CUCAUGGAc -3' miRNA: 3'- -GGCGGCa-CuaGUCCGCcGAGUAUCUc -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 126540 | 0.66 | 0.919812 |
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Target: 5'- cUCGCCG-GAUCcGGCuaccGGCUCAccgguagcggAGAGg -3' miRNA: 3'- -GGCGGCaCUAGuCCG----CCGAGUa---------UCUC- -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 129608 | 0.7 | 0.714591 |
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Target: 5'- gCGaCCGUGGagcucgUCcGGCGGCUCGUGGc- -3' miRNA: 3'- gGC-GGCACU------AGuCCGCCGAGUAUCuc -5' |
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| 25508 | 5' | -55.6 | NC_005337.1 | + | 130827 | 1.1 | 0.002659 |
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Target: 5'- gCCGCCGUGAUCAGGCGGCUCAUAGAGc -3' miRNA: 3'- -GGCGGCACUAGUCCGCCGAGUAUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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