Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25511 | 3' | -55.3 | NC_005337.1 | + | 36348 | 0.66 | 0.920066 |
Target: 5'- cGUCCGCGcGUCcGAgUGCGacucGCGACAc -3' miRNA: 3'- -CAGGCGCuCAGaCU-GCGCa---UGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 22640 | 0.66 | 0.920066 |
Target: 5'- aUCCGCGAGgccuucGGCGCGgacgcGCGGCu- -3' miRNA: 3'- cAGGCGCUCaga---CUGCGCa----UGCUGua -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 62724 | 0.66 | 0.920066 |
Target: 5'- uGUCCGCGAugaaGUC---CGUGUACGACu- -3' miRNA: 3'- -CAGGCGCU----CAGacuGCGCAUGCUGua -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 86392 | 0.66 | 0.920066 |
Target: 5'- uGUCCGCcAGgcggCUGcGCGCGUcCGGCGc -3' miRNA: 3'- -CAGGCGcUCa---GAC-UGCGCAuGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 115580 | 0.66 | 0.914245 |
Target: 5'- -gCCGCGuGUCUGAgCGUGUACu---- -3' miRNA: 3'- caGGCGCuCAGACU-GCGCAUGcugua -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 110361 | 0.66 | 0.90818 |
Target: 5'- -gCCGCGGGcgCgcgGGCGCGcggcaGCGACAg -3' miRNA: 3'- caGGCGCUCa-Ga--CUGCGCa----UGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 115519 | 0.66 | 0.901873 |
Target: 5'- -cCCuCGAGUCcauCGUGUACGGCAUg -3' miRNA: 3'- caGGcGCUCAGacuGCGCAUGCUGUA- -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 120557 | 0.66 | 0.901873 |
Target: 5'- aGUCCucGCgGAGcUCgGugGUGUGCGGCAa -3' miRNA: 3'- -CAGG--CG-CUC-AGaCugCGCAUGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 84894 | 0.66 | 0.901873 |
Target: 5'- -gCCGCGGGg--GGCG-GUGCGGCGg -3' miRNA: 3'- caGGCGCUCagaCUGCgCAUGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 107049 | 0.66 | 0.888543 |
Target: 5'- uGUCCGaCGAGgacCUGGCGgCG-GCGGCGc -3' miRNA: 3'- -CAGGC-GCUCa--GACUGC-GCaUGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 46133 | 0.66 | 0.888543 |
Target: 5'- gGUCCGCGAGcUCcGACGCcaUGgGGCGg -3' miRNA: 3'- -CAGGCGCUC-AGaCUGCGc-AUgCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 7463 | 0.66 | 0.888543 |
Target: 5'- cGUCCGUGGcGaCgagGGCGCGcACGACGUc -3' miRNA: 3'- -CAGGCGCU-CaGa--CUGCGCaUGCUGUA- -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 25309 | 0.66 | 0.888543 |
Target: 5'- -gCCGCGcGUCgaccGGCGCGUguACGACc- -3' miRNA: 3'- caGGCGCuCAGa---CUGCGCA--UGCUGua -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 109758 | 0.67 | 0.866817 |
Target: 5'- cGUCCGCGAgGUCgucCGCGgugggACGACc- -3' miRNA: 3'- -CAGGCGCU-CAGacuGCGCa----UGCUGua -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 35113 | 0.67 | 0.85124 |
Target: 5'- cGUCCGCGcGgcgCgGACGCccaGUGCGGCGc -3' miRNA: 3'- -CAGGCGCuCa--GaCUGCG---CAUGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 68395 | 0.67 | 0.843141 |
Target: 5'- cGUCCGCc---CUGcGCGCGUGCGugGUa -3' miRNA: 3'- -CAGGCGcucaGAC-UGCGCAUGCugUA- -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 42226 | 0.68 | 0.834845 |
Target: 5'- cGUCCGCGAGUU---CGCGcACGGCc- -3' miRNA: 3'- -CAGGCGCUCAGacuGCGCaUGCUGua -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 76533 | 0.68 | 0.827217 |
Target: 5'- -aCCGCGGGUCgucguaguugaugaaGGCGCaGUugGGCAc -3' miRNA: 3'- caGGCGCUCAGa--------------CUGCG-CAugCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 8069 | 0.68 | 0.808852 |
Target: 5'- cUCCGCGAccgCgagGACGCGcgACGGCAc -3' miRNA: 3'- cAGGCGCUca-Ga--CUGCGCa-UGCUGUa -5' |
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25511 | 3' | -55.3 | NC_005337.1 | + | 109390 | 0.68 | 0.807064 |
Target: 5'- --gCGCGGGUCUGGCGUGccguccacagacACGGCGUg -3' miRNA: 3'- cagGCGCUCAGACUGCGCa-----------UGCUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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