Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25540 | 3' | -64.1 | NC_005337.1 | + | 11389 | 0.66 | 0.496713 |
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Target: 5'- cGGCGGGcugaUGCgCCaggGCCCCg--GGuUCCGCa -3' miRNA: 3'- -CUGCCC----ACG-GG---CGGGGagaCC-AGGCG- -5' |
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| 25540 | 3' | -64.1 | NC_005337.1 | + | 57873 | 0.66 | 0.496713 |
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Target: 5'- gGACGGGcGCCaCGCCCCgcgCgcacGGUguacaacuaccUCGCg -3' miRNA: 3'- -CUGCCCaCGG-GCGGGGa--Ga---CCA-----------GGCG- -5' |
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| 25540 | 3' | -64.1 | NC_005337.1 | + | 23546 | 0.66 | 0.505906 |
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Target: 5'- cGGCGGG-GCCUGCCCCgc--GUacaCGCc -3' miRNA: 3'- -CUGCCCaCGGGCGGGGagacCAg--GCG- -5' |
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| 25540 | 3' | -64.1 | NC_005337.1 | + | 120440 | 0.66 | 0.515171 |
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Target: 5'- cGGCGGacUGCgCGCCCCacaUGGUCCu- -3' miRNA: 3'- -CUGCCc-ACGgGCGGGGag-ACCAGGcg -5' |
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| 25540 | 3' | -64.1 | NC_005337.1 | + | 17299 | 0.66 | 0.515171 |
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Target: 5'- --aGGauGUGCCCGCCCUcCUcGUCCuGCa -3' miRNA: 3'- cugCC--CACGGGCGGGGaGAcCAGG-CG- -5' |
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| 25540 | 3' | -64.1 | NC_005337.1 | + | 76405 | 0.66 | 0.543357 |
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Target: 5'- cGCGGGgcgcuggcGCCCGUCCUccUUGGUCaGCg -3' miRNA: 3'- cUGCCCa-------CGGGCGGGGa-GACCAGgCG- -5' |
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| 25540 | 3' | -64.1 | NC_005337.1 | + | 45316 | 0.66 | 0.543357 |
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Target: 5'- -cCGGGaccgcGCCCuCCgCCUCcgcgcGGUCCGCg -3' miRNA: 3'- cuGCCCa----CGGGcGG-GGAGa----CCAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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