Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
256 | 5' | -64.7 | AC_000008.1 | + | 17838 | 0.66 | 0.202862 |
Target: 5'- aUCGCCGCgGCgauuggCGCCGuGCCCGGaauuGCa -3' miRNA: 3'- cAGCGGUGgCG------GCGGC-UGGGCCg---UGa -5' |
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256 | 5' | -64.7 | AC_000008.1 | + | 33229 | 0.66 | 0.208162 |
Target: 5'- -cUGCUGCCGCCGCCGcUCCGuccuGCAg- -3' miRNA: 3'- caGCGGUGGCGGCGGCuGGGC----CGUga -5' |
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256 | 5' | -64.7 | AC_000008.1 | + | 23802 | 0.66 | 0.213034 |
Target: 5'- aUCGCCGCUGCCcgugccaGCCagGGCCCuuugcaGGCugUg -3' miRNA: 3'- cAGCGGUGGCGG-------CGG--CUGGG------CCGugA- -5' |
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256 | 5' | -64.7 | AC_000008.1 | + | 3585 | 0.66 | 0.213581 |
Target: 5'- uUUGCagcaGCCGCCGCCG-CCaUGaGCACc -3' miRNA: 3'- cAGCGg---UGGCGGCGGCuGG-GC-CGUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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