Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25981 | 3' | -55.1 | NC_005340.1 | + | 5209 | 0.66 | 0.578337 |
Target: 5'- gCAUCCGUgacGCCAUuGUCAagcgucaGGCGCu -3' miRNA: 3'- -GUAGGCGac-UGGUGuUAGUgg-----CCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 19186 | 0.66 | 0.578337 |
Target: 5'- -cUCCuGUgUGGCCu---UCACCGGUGCa -3' miRNA: 3'- guAGG-CG-ACUGGuguuAGUGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 10499 | 0.66 | 0.555711 |
Target: 5'- uGUCugCGCUGauugacauGCCGCAAaacCCGGCGCu -3' miRNA: 3'- gUAG--GCGAC--------UGGUGUUaguGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 11840 | 0.66 | 0.555711 |
Target: 5'- uCAUUCaCUG-CCACAAggggacUAUCGGCGCa -3' miRNA: 3'- -GUAGGcGACuGGUGUUa-----GUGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 445 | 0.66 | 0.555711 |
Target: 5'- cCAgugCCGCcGACCugAuggacgcUCACCGcGUGCc -3' miRNA: 3'- -GUa--GGCGaCUGGugUu------AGUGGC-CGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 16878 | 0.66 | 0.533349 |
Target: 5'- --cCCGCaUGACCuaccccGCAcUCACC-GCGCa -3' miRNA: 3'- guaGGCG-ACUGG------UGUuAGUGGcCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 4966 | 0.66 | 0.53113 |
Target: 5'- uCAUCCGCguuucugaaaacGAUUACAuUCuGCCGGuCGCa -3' miRNA: 3'- -GUAGGCGa-----------CUGGUGUuAG-UGGCC-GCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 28787 | 0.66 | 0.522287 |
Target: 5'- aCAUCUGacuaccgGcAUCACAAUCAgCCGGgGCg -3' miRNA: 3'- -GUAGGCga-----C-UGGUGUUAGU-GGCCgCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 6471 | 0.67 | 0.468461 |
Target: 5'- ----aGCUcGACCGCAAU-ACCGGUGUg -3' miRNA: 3'- guaggCGA-CUGGUGUUAgUGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 28292 | 0.67 | 0.468461 |
Target: 5'- --aCgGUaGACC---GUCACCGGCGCa -3' miRNA: 3'- guaGgCGaCUGGuguUAGUGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 884 | 0.68 | 0.458035 |
Target: 5'- --aCUGCUGACUucaccgGCAAaauaCAcCCGGCGCg -3' miRNA: 3'- guaGGCGACUGG------UGUUa---GU-GGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 20697 | 0.68 | 0.458035 |
Target: 5'- --aCCGCUGGCCGuCAGcgcacUACCGGCu- -3' miRNA: 3'- guaGGCGACUGGU-GUUa----GUGGCCGcg -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 13146 | 0.68 | 0.441616 |
Target: 5'- aAUCCGUgGACUACGgcggaguuuAUCGCCuggcuggagaguugcGGCGCa -3' miRNA: 3'- gUAGGCGaCUGGUGU---------UAGUGG---------------CCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 12981 | 0.69 | 0.398253 |
Target: 5'- ---aCGCUGGCCGCAA-CGCCGaaaGCg -3' miRNA: 3'- guagGCGACUGGUGUUaGUGGCcg-CG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 5428 | 0.69 | 0.388784 |
Target: 5'- cCGUCCGCgucgGugauguugcucAUCACGcgCGCCGGgGCu -3' miRNA: 3'- -GUAGGCGa---C-----------UGGUGUuaGUGGCCgCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 2160 | 0.69 | 0.387846 |
Target: 5'- cCAUCCGCaaaaccaUGACCAgCA---GCgGGCGCa -3' miRNA: 3'- -GUAGGCG-------ACUGGU-GUuagUGgCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 9860 | 0.69 | 0.379465 |
Target: 5'- --cUgGCUGACCACccgCGCCGGuCGUu -3' miRNA: 3'- guaGgCGACUGGUGuuaGUGGCC-GCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 14384 | 0.69 | 0.379465 |
Target: 5'- uGUCUGCUGAaaAUAAUUGCUGGCauGCa -3' miRNA: 3'- gUAGGCGACUggUGUUAGUGGCCG--CG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 15233 | 0.7 | 0.352419 |
Target: 5'- -cUCUcCUGACUGCucaggccGUCACCGGCGUc -3' miRNA: 3'- guAGGcGACUGGUGu------UAGUGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 16942 | 0.7 | 0.347176 |
Target: 5'- --gUCGCUGGCCGCuAaggugauugguuuuuGUCACCGGCu- -3' miRNA: 3'- guaGGCGACUGGUG-U---------------UAGUGGCCGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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