Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25981 | 3' | -55.1 | NC_005340.1 | + | 8201 | 0.7 | 0.310448 |
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Target: 5'- -cUCgCGCUGGCCGua---ACCGGCGCc -3' miRNA: 3'- guAG-GCGACUGGUguuagUGGCCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 2843 | 0.72 | 0.268085 |
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Target: 5'- cCAgugCUGCUGACCGCAucgaaaucgacaucaGUCACa-GCGCg -3' miRNA: 3'- -GUa--GGCGACUGGUGU---------------UAGUGgcCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 10120 | 0.73 | 0.225523 |
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Target: 5'- --gCCGCUGACCGuCAGcgCACCGGUuuccgGCu -3' miRNA: 3'- guaGGCGACUGGU-GUUa-GUGGCCG-----CG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 7806 | 0.73 | 0.212861 |
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Target: 5'- cCAUCCGCUGacgaaaaaccgugGCUACGacgucauuGUCACCGGCu- -3' miRNA: 3'- -GUAGGCGAC-------------UGGUGU--------UAGUGGCCGcg -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 8633 | 1.11 | 0.000341 |
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Target: 5'- gCAUCCGCUGACCACAAUCACCGGCGCu -3' miRNA: 3'- -GUAGGCGACUGGUGUUAGUGGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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