miRNA: 3'- guaGGCGACUGGUG-U---------------UAGUGGCCGcg -5'
miRNA: 3'- guAGG-CG-ACUGGuguuAGUGGCCGCG- -5'
miRNA: 3'- guaGGCGACUGGU-GUUa----GUGGCCGcg -5'
miRNA: 3'- guaGgCGaCUGGuguUAGUGGCCGCG- -5'
miRNA: 3'- -GUAGGCga-----C-UGGUGUUAGU-GGCCgCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25981 | 3' | -55.1 | NC_005340.1 | + | 16942 | 0.7 | 0.347176 |
|
Target: 5'- --gUCGCUGGCCGCuAaggugauugguuuuuGUCACCGGCu- -3' miRNA: 3'- guaGGCGACUGGUG-U---------------UAGUGGCCGcg -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 19186 | 0.66 | 0.578337 |
|
Target: 5'- -cUCCuGUgUGGCCu---UCACCGGUGCa -3' miRNA: 3'- guAGG-CG-ACUGGuguuAGUGGCCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 20697 | 0.68 | 0.458035 |
|
Target: 5'- --aCCGCUGGCCGuCAGcgcacUACCGGCu- -3' miRNA: 3'- guaGGCGACUGGU-GUUa----GUGGCCGcg -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 28292 | 0.67 | 0.468461 |
|
Target: 5'- --aCgGUaGACC---GUCACCGGCGCa -3' miRNA: 3'- guaGgCGaCUGGuguUAGUGGCCGCG- -5' |
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| 25981 | 3' | -55.1 | NC_005340.1 | + | 28787 | 0.66 | 0.522287 |
|
Target: 5'- aCAUCUGacuaccgGcAUCACAAUCAgCCGGgGCg -3' miRNA: 3'- -GUAGGCga-----C-UGGUGUUAGU-GGCCgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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