Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25981 | 3' | -55.1 | NC_005340.1 | + | 445 | 0.66 | 0.555711 |
Target: 5'- cCAgugCCGCcGACCugAuggacgcUCACCGcGUGCc -3' miRNA: 3'- -GUa--GGCGaCUGGugUu------AGUGGC-CGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 10499 | 0.66 | 0.555711 |
Target: 5'- uGUCugCGCUGauugacauGCCGCAAaacCCGGCGCu -3' miRNA: 3'- gUAG--GCGAC--------UGGUGUUaguGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 11840 | 0.66 | 0.555711 |
Target: 5'- uCAUUCaCUG-CCACAAggggacUAUCGGCGCa -3' miRNA: 3'- -GUAGGcGACuGGUGUUa-----GUGGCCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 5209 | 0.66 | 0.578337 |
Target: 5'- gCAUCCGUgacGCCAUuGUCAagcgucaGGCGCu -3' miRNA: 3'- -GUAGGCGac-UGGUGuUAGUgg-----CCGCG- -5' |
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25981 | 3' | -55.1 | NC_005340.1 | + | 19186 | 0.66 | 0.578337 |
Target: 5'- -cUCCuGUgUGGCCu---UCACCGGUGCa -3' miRNA: 3'- guAGG-CG-ACUGGuguuAGUGGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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