Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26032 | 3' | -52.4 | NC_005342.2 | + | 22904 | 0.66 | 0.834563 |
Target: 5'- aGAAGCGcGGUGcugccgcucgaGUUGUGCAGCGuAUa -3' miRNA: 3'- gCUUCGCuUCAC-----------CGACGCGUUGCuUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 40118 | 0.66 | 0.819507 |
Target: 5'- uGggGCGAgcgcgagcuGGaaaUGGCcgGCGCAggccgcgucgaucugGCGAGCg -3' miRNA: 3'- gCuuCGCU---------UC---ACCGa-CGCGU---------------UGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 41100 | 0.66 | 0.815657 |
Target: 5'- uCGccGCGAuccgcucgaUGGCUGCGCAAUGcaguGCg -3' miRNA: 3'- -GCuuCGCUuc-------ACCGACGCGUUGCu---UG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 36406 | 0.66 | 0.815657 |
Target: 5'- uCGAcaaucAGCGcauGUGGCaaucgcgucUGCGCAacGCGGGCu -3' miRNA: 3'- -GCU-----UCGCuu-CACCG---------ACGCGU--UGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 21018 | 0.66 | 0.815657 |
Target: 5'- aCGGGcGaCGAAGUGGCcGCGCAgugcugcuccuGgGGACu -3' miRNA: 3'- -GCUU-C-GCUUCACCGaCGCGU-----------UgCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 38771 | 0.67 | 0.775453 |
Target: 5'- gGAuGCGAAGUcGG-UGCGCAccGCGAu- -3' miRNA: 3'- gCUuCGCUUCA-CCgACGCGU--UGCUug -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 27548 | 0.67 | 0.764973 |
Target: 5'- cCGggGCGAAu---CUGCGCAuUGAACa -3' miRNA: 3'- -GCuuCGCUUcaccGACGCGUuGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 44818 | 0.67 | 0.762859 |
Target: 5'- gCGAGGCGAuacucgacgccUGuCUGCGCGGCGAucGCg -3' miRNA: 3'- -GCUUCGCUuc---------ACcGACGCGUUGCU--UG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 41391 | 0.67 | 0.754347 |
Target: 5'- gCGgcGCGccGUGGCgGCGauGCGGGCu -3' miRNA: 3'- -GCuuCGCuuCACCGaCGCguUGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 29549 | 0.67 | 0.732705 |
Target: 5'- cCGAucGCGAGGc-GUUGCGCuuCGGACg -3' miRNA: 3'- -GCUu-CGCUUCacCGACGCGuuGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 38397 | 0.68 | 0.699461 |
Target: 5'- cCGGcuGGCGcugaAGGUGGCg--GCAACGAACu -3' miRNA: 3'- -GCU--UCGC----UUCACCGacgCGUUGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 26068 | 0.68 | 0.688222 |
Target: 5'- aGAacGGCGcccaauuGG-GGCuaUGCGCAGCGAACc -3' miRNA: 3'- gCU--UCGCu------UCaCCG--ACGCGUUGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 47011 | 0.68 | 0.676926 |
Target: 5'- aGAAgGCGAAGUGcgcgcgaauGCUGCGCcGCaAGCa -3' miRNA: 3'- gCUU-CGCUUCAC---------CGACGCGuUGcUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 6275 | 0.69 | 0.665583 |
Target: 5'- aCGAGcGCGcuGGUgaaaGGCUGCGCAGCcgugaGGACg -3' miRNA: 3'- -GCUU-CGCu-UCA----CCGACGCGUUG-----CUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 41018 | 0.69 | 0.654208 |
Target: 5'- aCGAGGCcGAGcucgcccGGC-GCGCGAUGGACg -3' miRNA: 3'- -GCUUCGcUUCa------CCGaCGCGUUGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 29596 | 0.7 | 0.563332 |
Target: 5'- uCGucGGCGAAGc-GCUGCGCGAuCGGGCc -3' miRNA: 3'- -GCu-UCGCUUCacCGACGCGUU-GCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 39191 | 0.7 | 0.563332 |
Target: 5'- -aAGGCGAAGaUGGaagcgcgcgGCGUAACGGACg -3' miRNA: 3'- gcUUCGCUUC-ACCga-------CGCGUUGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 8874 | 0.7 | 0.552142 |
Target: 5'- uGAucuuGGCGAGGuUGGCgucgaUGCGCGACGcGGCu -3' miRNA: 3'- gCU----UCGCUUC-ACCG-----ACGCGUUGC-UUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 21221 | 0.72 | 0.490949 |
Target: 5'- aCGggGCGccgacgcaaugggucAAGUuccuGGCcgGCGCGGCGGGCa -3' miRNA: 3'- -GCuuCGC---------------UUCA----CCGa-CGCGUUGCUUG- -5' |
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26032 | 3' | -52.4 | NC_005342.2 | + | 30558 | 0.73 | 0.42515 |
Target: 5'- ---cGCGucGUGGCcGCGCuguGCGAGCa -3' miRNA: 3'- gcuuCGCuuCACCGaCGCGu--UGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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