miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2614 3' -52.4 NC_001488.1 + 4372 0.66 0.339089
Target:  5'- uGGUGUGG-CGCCaaAAGaGACuAGAGCCc -3'
miRNA:   3'- uCCGCACCuGUGGg-UUC-CUG-UUUUGG- -5'
2614 3' -52.4 NC_001488.1 + 380 0.67 0.276077
Target:  5'- gAGGa-UGGAUcCCCAAGGugAGucuccguGACCa -3'
miRNA:   3'- -UCCgcACCUGuGGGUUCCugUU-------UUGG- -5'
2614 3' -52.4 NC_001488.1 + 8561 0.67 0.276077
Target:  5'- gAGGa-UGGAUcCCCAAGGugAGucuccguGACCa -3'
miRNA:   3'- -UCCgcACCUGuGGGUUCCugUU-------UUGG- -5'
2614 3' -52.4 NC_001488.1 + 4327 0.7 0.171279
Target:  5'- gAGGcCGUGGAgGCCUuGGGGCGucaggggaacaagggGAGCUa -3'
miRNA:   3'- -UCC-GCACCUgUGGGuUCCUGU---------------UUUGG- -5'
2614 3' -52.4 NC_001488.1 + 4158 0.7 0.169113
Target:  5'- cAGGCGgccuuGcCACCCcuacugcAGGGCAAGACCa -3'
miRNA:   3'- -UCCGCac---CuGUGGGu------UCCUGUUUUGG- -5'
2614 3' -52.4 NC_001488.1 + 3625 0.71 0.144046
Target:  5'- cAGGCcaGUaGGugAcCCCAAGGACAuAAACUg -3'
miRNA:   3'- -UCCG--CA-CCugU-GGGUUCCUGU-UUUGG- -5'
2614 3' -52.4 NC_001488.1 + 1820 1.13 0.000071
Target:  5'- cAGGCGUGGACACCCAAGGACAAAACCa -3'
miRNA:   3'- -UCCGCACCUGUGGGUUCCUGUUUUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.