Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2624 | 3' | -65.3 | NC_001491.2 | + | 3326 | 0.66 | 0.516349 |
Target: 5'- gGCCgaggCCCGgGCCCgGgCCCCCUCGg-- -3' miRNA: 3'- gCGG----GGGUaUGGGgC-GGGGGAGCgag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 35382 | 0.66 | 0.498067 |
Target: 5'- gCGCgCCUGUACCCggacucugCGCCCCUgaggcugugcCGCUCc -3' miRNA: 3'- -GCGgGGGUAUGGG--------GCGGGGGa---------GCGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 91205 | 0.66 | 0.498067 |
Target: 5'- -aCCCaCgCAUGCCCCgaagucaaacGCUCCCUCGC-Cg -3' miRNA: 3'- gcGGG-G-GUAUGGGG----------CGGGGGAGCGaG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 46070 | 0.66 | 0.489035 |
Target: 5'- aCGCCuCCCGgACCCU--CCCCUCGUcCa -3' miRNA: 3'- -GCGG-GGGUaUGGGGcgGGGGAGCGaG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 32446 | 0.66 | 0.489035 |
Target: 5'- cCGCUCCCAgccGCCCgggaggagGCCUCCUCcCUCc -3' miRNA: 3'- -GCGGGGGUa--UGGGg-------CGGGGGAGcGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 33435 | 0.66 | 0.480081 |
Target: 5'- aGCCCgag-GCCCCGgCgCCCUCGgUCc -3' miRNA: 3'- gCGGGgguaUGGGGCgG-GGGAGCgAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 19572 | 0.66 | 0.471207 |
Target: 5'- -cCCCCCugagggcUACCCCGacgaCCCCCUgCGCg- -3' miRNA: 3'- gcGGGGGu------AUGGGGC----GGGGGA-GCGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 51305 | 0.66 | 0.462418 |
Target: 5'- uCGCCUUCGcgGCUCUGCCggCCC-CGCUCu -3' miRNA: 3'- -GCGGGGGUa-UGGGGCGG--GGGaGCGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 121323 | 0.67 | 0.453715 |
Target: 5'- uGCCCCgAUAUCgaGCaCCUgcgCUCGCUCa -3' miRNA: 3'- gCGGGGgUAUGGggCG-GGG---GAGCGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 148443 | 0.67 | 0.445102 |
Target: 5'- aCGCCCgCGgccGCUCUGCCCCg-CGUUCc -3' miRNA: 3'- -GCGGGgGUa--UGGGGCGGGGgaGCGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 8684 | 0.67 | 0.445102 |
Target: 5'- gCGCCCcgCCGUggcGCCCCGaugugaaCCgCCUCGCg- -3' miRNA: 3'- -GCGGG--GGUA---UGGGGCg------GG-GGAGCGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 35798 | 0.67 | 0.434044 |
Target: 5'- cCGCCgCCGaGCCCCGCgggucgacugggagCCCggguucggcuCUCGCUCc -3' miRNA: 3'- -GCGGgGGUaUGGGGCG--------------GGG----------GAGCGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 35642 | 0.67 | 0.428156 |
Target: 5'- gCGCCCCgUGUACCUCGCCUgCggccggCGCg- -3' miRNA: 3'- -GCGGGG-GUAUGGGGCGGGgGa-----GCGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 34099 | 0.67 | 0.419827 |
Target: 5'- cCGCCCCUGUGgcCCaCCGUCCCCgggaaGCa- -3' miRNA: 3'- -GCGGGGGUAU--GG-GGCGGGGGag---CGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 40829 | 0.68 | 0.39544 |
Target: 5'- aGCCCCCcuuggACgaCUGCCCCC-CGCg- -3' miRNA: 3'- gCGGGGGua---UGg-GGCGGGGGaGCGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 40562 | 0.68 | 0.39544 |
Target: 5'- uGCCCCauuCAUGCUaCGCCCCCUCcCa- -3' miRNA: 3'- gCGGGG---GUAUGGgGCGGGGGAGcGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 35960 | 0.68 | 0.364382 |
Target: 5'- aGCCCCgGcgugGCCCagGCCCuCCgccgCGCUCc -3' miRNA: 3'- gCGGGGgUa---UGGGg-CGGG-GGa---GCGAG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 29453 | 0.69 | 0.349499 |
Target: 5'- aCGCCCCCAccCCCCGUCUCgggagCGCg- -3' miRNA: 3'- -GCGGGGGUauGGGGCGGGGga---GCGag -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 122095 | 0.69 | 0.342221 |
Target: 5'- uGCCCCCAaaccauCCCCGagcuuUCCUCUCGCcuUCa -3' miRNA: 3'- gCGGGGGUau----GGGGC-----GGGGGAGCG--AG- -5' |
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2624 | 3' | -65.3 | NC_001491.2 | + | 27581 | 0.69 | 0.327996 |
Target: 5'- aCGCuuuCCCCcgACCUCGCCCCgagUCGCg- -3' miRNA: 3'- -GCG---GGGGuaUGGGGCGGGGg--AGCGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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