Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26264 | 5' | -50.5 | NC_005345.2 | + | 45200 | 0.67 | 0.877438 |
Target: 5'- cCGCCCGAGagGCUGC-CGGccgCggCGAGc -3' miRNA: 3'- aGUGGGCUU--UGACGaGCUa--GaaGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 9533 | 0.67 | 0.885289 |
Target: 5'- -gACCCGGAGCUGUUCcacccggaGAUCgggCGGa -3' miRNA: 3'- agUGGGCUUUGACGAG--------CUAGaa-GCUc -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 26138 | 0.67 | 0.892861 |
Target: 5'- uUCGCCCGGA--UGCcCGAccUCggCGAGg -3' miRNA: 3'- -AGUGGGCUUugACGaGCU--AGaaGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 27509 | 0.67 | 0.892861 |
Target: 5'- cCGCCCGAGccGCUGaggaUCGAUCUcccgccCGAu -3' miRNA: 3'- aGUGGGCUU--UGACg---AGCUAGAa-----GCUc -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 17973 | 0.66 | 0.900149 |
Target: 5'- cCugCCGGGccGCgGcCUCGAUCUcccgugUCGAGg -3' miRNA: 3'- aGugGGCUU--UGaC-GAGCUAGA------AGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 10932 | 0.66 | 0.900149 |
Target: 5'- -aGCCCGuAGCgucccGCUCGAUCgacUCGGu -3' miRNA: 3'- agUGGGCuUUGa----CGAGCUAGa--AGCUc -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 6495 | 0.66 | 0.902983 |
Target: 5'- -aGCCCGAGGCguggacgccgcaggGCUCGAagaUCGAGc -3' miRNA: 3'- agUGGGCUUUGa-------------CGAGCUagaAGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 22982 | 0.66 | 0.907148 |
Target: 5'- aCACCCGcGAACUcggGCUCGGgguccUCgcCGAGg -3' miRNA: 3'- aGUGGGC-UUUGA---CGAGCU-----AGaaGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 21107 | 0.66 | 0.913853 |
Target: 5'- aCACCCGccGAaaGCUCGA-CgacgUCGAGg -3' miRNA: 3'- aGUGGGCu-UUgaCGAGCUaGa---AGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 41075 | 0.66 | 0.926376 |
Target: 5'- -aGCCCGGccgcCUGCUCGG-CggCGAGc -3' miRNA: 3'- agUGGGCUuu--GACGAGCUaGaaGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 32513 | 0.66 | 0.926376 |
Target: 5'- cUCugCCGGcgGGCgGCcCGAUCUgcgCGGGu -3' miRNA: 3'- -AGugGGCU--UUGaCGaGCUAGAa--GCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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