Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26264 | 5' | -50.5 | NC_005345.2 | + | 35569 | 0.69 | 0.785366 |
Target: 5'- cUCGCCCGGGACg---CGAUCU-CGAa -3' miRNA: 3'- -AGUGGGCUUUGacgaGCUAGAaGCUc -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 35720 | 0.69 | 0.764528 |
Target: 5'- aCGCgCCGAAACUGCUCGcccgCggCGGc -3' miRNA: 3'- aGUG-GGCUUUGACGAGCua--GaaGCUc -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 39273 | 0.69 | 0.805518 |
Target: 5'- -gGCCCGcuGCUGCUCG----UCGAGc -3' miRNA: 3'- agUGGGCuuUGACGAGCuagaAGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 39656 | 0.7 | 0.721197 |
Target: 5'- -gGCCCGcGACUGCcacUCGGUCUcagcCGAGu -3' miRNA: 3'- agUGGGCuUUGACG---AGCUAGAa---GCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 39707 | 0.68 | 0.815306 |
Target: 5'- cCGCCCGGGACgaGCUCGggCcaCGAc -3' miRNA: 3'- aGUGGGCUUUGa-CGAGCuaGaaGCUc -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 41075 | 0.66 | 0.926376 |
Target: 5'- -aGCCCGGccgcCUGCUCGG-CggCGAGc -3' miRNA: 3'- agUGGGCUuu--GACGAGCUaGaaGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 42055 | 0.7 | 0.753883 |
Target: 5'- -aGCCCGAGuuaccgGCUGCUCGG-CgacagcgUCGAGc -3' miRNA: 3'- agUGGGCUU------UGACGAGCUaGa------AGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 42255 | 0.73 | 0.585192 |
Target: 5'- uUCACCUGucGCUGCUCGAUCcacaugCGcAGc -3' miRNA: 3'- -AGUGGGCuuUGACGAGCUAGaa----GC-UC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 42627 | 1.09 | 0.002726 |
Target: 5'- aUCACCCGAAACUGCUCGAUCUUCGAGc -3' miRNA: 3'- -AGUGGGCUUUGACGAGCUAGAAGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 44804 | 0.72 | 0.642182 |
Target: 5'- cCGCCCGAGGCaggaccccgGCUCGGcCgaugUCGAGa -3' miRNA: 3'- aGUGGGCUUUGa--------CGAGCUaGa---AGCUC- -5' |
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26264 | 5' | -50.5 | NC_005345.2 | + | 45200 | 0.67 | 0.877438 |
Target: 5'- cCGCCCGAGagGCUGC-CGGccgCggCGAGc -3' miRNA: 3'- aGUGGGCUU--UGACGaGCUa--GaaGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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