Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2629 | 3' | -55.9 | NC_001491.2 | + | 18084 | 0.66 | 0.909415 |
Target: 5'- cAGAAUGUGCaGGgGCuGCCAGUacaAGUc -3' miRNA: 3'- cUUUUGCACGgCCgCG-CGGUCAg--UCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 2531 | 0.66 | 0.909415 |
Target: 5'- cGAAggUGagcGCCGG-GCGCCAGgccUCGGg -3' miRNA: 3'- -CUUuuGCa--CGGCCgCGCGGUC---AGUCg -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 95536 | 0.66 | 0.903217 |
Target: 5'- aGAAcuCGUGCgCGcaaugguggacuGCGCGCaCAGcUCGGCu -3' miRNA: 3'- -CUUuuGCACG-GC------------CGCGCG-GUC-AGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 3685 | 0.66 | 0.896783 |
Target: 5'- uGAAGGCGgacgGCCuGgGCGCCcuGGUCcccggAGCg -3' miRNA: 3'- -CUUUUGCa---CGGcCgCGCGG--UCAG-----UCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 2181 | 0.67 | 0.876099 |
Target: 5'- cGAGACGU-CCGGgGCcCCGGUcCAGUu -3' miRNA: 3'- cUUUUGCAcGGCCgCGcGGUCA-GUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 71588 | 0.67 | 0.868758 |
Target: 5'- uAAGAgGcGCUGGCGCacGCCAG-CAGUu -3' miRNA: 3'- cUUUUgCaCGGCCGCG--CGGUCaGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 68539 | 0.67 | 0.868758 |
Target: 5'- -uAGACGUGCUgggGGCGaCGCUGGcaccgacaUCGGCu -3' miRNA: 3'- cuUUUGCACGG---CCGC-GCGGUC--------AGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 119347 | 0.67 | 0.85344 |
Target: 5'- aGGAAcCG-GCUauGGCGCGCguguuGGUCAGCg -3' miRNA: 3'- -CUUUuGCaCGG--CCGCGCGg----UCAGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 144467 | 0.67 | 0.845473 |
Target: 5'- -cGAGCG-GCCGGCGUuugGCCA-UgGGCg -3' miRNA: 3'- cuUUUGCaCGGCCGCG---CGGUcAgUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 134861 | 0.67 | 0.837312 |
Target: 5'- ---cAC-UGCaCGGCGCGCUAGUUuuauaGGCu -3' miRNA: 3'- cuuuUGcACG-GCCGCGCGGUCAG-----UCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 31844 | 0.68 | 0.828962 |
Target: 5'- cGAAGCGgucGCCcucGGC-CGCCGG-CAGCg -3' miRNA: 3'- cUUUUGCa--CGG---CCGcGCGGUCaGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 90046 | 0.68 | 0.820431 |
Target: 5'- ----cCGUGCCGGCGCucuuGCCGuuguGUCcuuGCa -3' miRNA: 3'- cuuuuGCACGGCCGCG----CGGU----CAGu--CG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 4819 | 0.68 | 0.793837 |
Target: 5'- uGGAGcCGgaGCgGGUGCGCCGGgggaagCGGCg -3' miRNA: 3'- -CUUUuGCa-CGgCCGCGCGGUCa-----GUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 77361 | 0.69 | 0.764016 |
Target: 5'- cGAGGCGUcuuuguggGCgGGCGCGCCuggcucggguucGUCAGUg -3' miRNA: 3'- cUUUUGCA--------CGgCCGCGCGGu-----------CAGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 81737 | 0.69 | 0.756359 |
Target: 5'- cGGAGCG-GCCGGUG-GCCAcgcgCAGCa -3' miRNA: 3'- cUUUUGCaCGGCCGCgCGGUca--GUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 39013 | 0.69 | 0.756359 |
Target: 5'- -cAGACGggggagaagGCCGGCuGCcccaggaccaGCUAGUCGGCg -3' miRNA: 3'- cuUUUGCa--------CGGCCG-CG----------CGGUCAGUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 5332 | 0.7 | 0.727057 |
Target: 5'- -----gGUGCCGGgGaCGCCGGcugggCGGCa -3' miRNA: 3'- cuuuugCACGGCCgC-GCGGUCa----GUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 69706 | 0.71 | 0.625394 |
Target: 5'- ---cGCGU-CCGGCGCGgCGGUugcCAGCa -3' miRNA: 3'- cuuuUGCAcGGCCGCGCgGUCA---GUCG- -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 25302 | 0.72 | 0.615117 |
Target: 5'- cGAGGugcCGUGCC-GCGUGCCGGUCGa- -3' miRNA: 3'- -CUUUu--GCACGGcCGCGCGGUCAGUcg -5' |
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2629 | 3' | -55.9 | NC_001491.2 | + | 68922 | 0.72 | 0.594611 |
Target: 5'- --cAACGcccGCCGGCGUgGCCGGcaUCAGCu -3' miRNA: 3'- cuuUUGCa--CGGCCGCG-CGGUC--AGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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