Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2629 | 3' | -55.9 | NC_001491.2 | + | 22979 | 0.72 | 0.564077 |
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Target: 5'- cAAGACGaUGCCGGCGCuCCAuauGUCuGCc -3' miRNA: 3'- cUUUUGC-ACGGCCGCGcGGU---CAGuCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 121613 | 0.73 | 0.514221 |
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Target: 5'- uGGAAACGacgcaGCCGcGCGCGCUAGguaugUAGCg -3' miRNA: 3'- -CUUUUGCa----CGGC-CGCGCGGUCa----GUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 10084 | 0.74 | 0.456939 |
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Target: 5'- ---cGCG-GUCGuGCGCGCCGuGUCGGCg -3' miRNA: 3'- cuuuUGCaCGGC-CGCGCGGU-CAGUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 30068 | 0.75 | 0.438612 |
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Target: 5'- uGggGugGgGCCGGCGCGUguGUguaUAGCg -3' miRNA: 3'- -CuuUugCaCGGCCGCGCGguCA---GUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 107436 | 0.76 | 0.386275 |
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Target: 5'- ----uCGUGCUGGCGUGUCAGgucCAGCc -3' miRNA: 3'- cuuuuGCACGGCCGCGCGGUCa--GUCG- -5' |
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| 2629 | 3' | -55.9 | NC_001491.2 | + | 144566 | 1.12 | 0.001772 |
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Target: 5'- gGAAAACGUGCCGGCGCGCCAGUCAGCg -3' miRNA: 3'- -CUUUUGCACGGCCGCGCGGUCAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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